Elevated expression of the human parainfluenza virus type 1 F gene downregulates HN expression

Virology
T BousseA Portner

Abstract

Interactions involved in the expression of parainfluenza glycoproteins were examined by expressing cDNA clones of the HN and F genes from human parainfluenza virus type-1 (hPIV1) or Sendai virus (SV) in recombinant Semliki Forest virus (recSFV) or vaccinia-T7 expression vectors. We found that expression of a cloned F protein gene of hPIV1 resulted in downregulation of the HN proteins of hPIV1 or SV. Compared to the amount of HN expressed in the absence of F, coexpression of HN and F led to about 70% reduction in HN. This reduction of HN was observed in both total cell lysates and in protein localized on the cell surface. In contrast to hPIV1 F, SV F did not suppress the expression of HN. Northern blot analysis indicated that similar levels of HN mRNA accumulated in the absence or presence of hPIV1 F. The reduction of HN protein expression by hPIV1 F was detectable after as little as a 10-min labeling period, suggesting that downregulation occurred at the level of translation or at an early stage of protein folding. In hPIV1-infected cells, the amount of F protein synthesized was only about 15% of that of HN, whereas SV F is expressed at high levels. When the level of F in hPIV1-infected cells was artificially increased by recSF...Continue Reading

References

Jun 1, 1992·Genetic Analysis, Techniques and Applications·T TakahashiA Portner
Feb 1, 1985·The Journal of General Virology·B M BlumbergD Kolakofsky
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
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Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R G PatersonR A Lamb
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Logan, T Shenk

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