Elevated formation of lipoxins in viral antibody-positive rat alveolar macrophages

American Journal of Respiratory Cell and Molecular Biology
S J Kim

Abstract

Alveolar macrophages (AM) obtained both from specific viral antibody-positive (VA+) rats and from specific viral antibody-negative (VA-) rats were compared for their respective capacity to produce lipoxins (LX) in a given experimental condition. Both cell preparations were incubated with (15S), 15-hydroxy-5,8,11-cis-13-trans-eicosatetraenoic acid (15-HETE) in the presence of calcium ionophore A23187. The reaction mixtures were extracted and chromatographed on reverse-phase high performance liquid chromatography (RP-HPLC). Profiles of LX formation in each group were evaluated on the basis of chromatographic behavior, UV spectral characteristics (lambda max at 301 nm with shoulders at 289 nm and 317 nm), and co-elution with synthetic lipoxin A4 (LXA4) and lipoxin B4 (LXB4) standards as well as with authentic materials. The AM harvested from VA+ rats (AM-VA+) produced consistently detectable levels of LX (i.e., LXA4 and LXB4), whereas the AM from VA- rats (AM-VA-) produced no detectable LX under the same experimental condition. These results offer a possibility that elevated capacity of the AM-VA+ to produce LX may be associated with previous exposures to selected specific viral pathogens and/or with viral infections. Considering ...Continue Reading

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Citations

May 15, 2003·British Journal of Pharmacology·Nan ChiangCharles N Serhan
Jan 10, 2014·Immunology·Clark D Russell, Jürgen Schwarze
Sep 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P G BardinP K Pattemore
Jan 1, 1996·Journal of Basic Microbiology·W Małek
Nov 30, 2020·BioFactors·Shayna Sandhaus, Andrew G Swick
Jan 30, 2021·FEMS Microbiology Reviews·Ebony A MonsonKarla J Helbig
Apr 14, 1994·Biochimica Et Biophysica Acta·C N Serhan

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