Elevated gene expression in chalcone synthase enzyme suggests an increased production of flavonoids in skin and synchronized red cell cultures of North American native grape berries.

DNA and Cell Biology
Gina DavisVioletka Tsolova

Abstract

Anthocyanins are antioxidants and are among the natural products synthesized via the flavonoid biosynthesis pathway. Anthocyanins have been recommended for dietary intake in the prevention of cardiovascular diseases, cancer, and age-related conditions such as Alzheimer's disease or dementia. With an increasingly aging population in many parts of the world, strategies for the commercial production of in vitro synchronized red cell cultures as natural antioxidants will be a significant contribution to human medicine. Red pigmented fruits such as grapes (Vitis sp.) are a major source of bioavailable anthocyanins and other polyphenols. Since the level of antioxidants varies among cultivars, this study is the first one that phytochemically and genetically characterizes native grape cultivars of North America to determine the optimal cultivar and berry cells for the production of anthocyanins as antioxidants. Using real-time PCR and bioinformatics approaches, we tested for the transcript expression of the chalcone synthase (CHS) gene, an enzyme involved in the flavonoid and anthocyanin biosynthesis pathway, in different parts of physiologically mature grape berries and in vitro synchronized red cells. A low level of expression was re...Continue Reading

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Citations

May 15, 2013·Journal of Agricultural and Food Chemistry·Cesar Nopo-OlazabalFabricio Medina-Bolivar
Jan 24, 2014·Nutrients·Vasil GeorgievVioleta Tsolova
May 11, 2016·Plant Physiology and Biochemistry : PPB·Huiling WangHaiying Xu
Apr 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrey S Marchev, Milen I Georgiev

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MEGA
Basic Local Alignment Tool ( BLAST )
Plotcon
BLAST
Coffee

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