Elevated glucose induces congenital heart defects by altering the expression of tbx5, tbx20, and has2 in developing zebrafish embryos

Birth Defects Research. Part A, Clinical and Molecular Teratology
Jintao LiangHouyan Song


Maternal diabetes increases the risk of congenital heart defects in infants, and hyperglycemia acts as a major teratogen. Multiple steps of cardiac development, including endocardial cushion morphogenesis and development of neural crest cells, are challenged under elevated glucose conditions. However, the direct effect of hyperglycemia on embryo heart organogenesis remains to be investigated. Zebrafish embryos in different stages were exposed to D-glucose for 12 or 24 hr to determine the sensitive window during early heart development. In the subsequent study, 6 hr post-fertilization embryos were treated with either 25 mmol/liter D-glucose or L-glucose for 24 hr. The expression of genes was analyzed by whole-mount in situ hybridization. The highest incidence of cardiac malformations was found during 6-30 hpf exposure periods. After 24 hr exposure, D-glucose-treated embryos exhibited significant developmental delay and diverse cardiac malformations, but embryos exposed to L-glucose showed no apparent phenotype. Further investigation of the origin of heart defects showed that cardiac looping was affected earliest, while the specification of cardiac progenitors and heart tube assembly were complete. Moreover, the expression patter...Continue Reading


Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
May 3, 1996·Science·E N Olson, D Srivastava
Oct 1, 1996·Teratology·E A ReeceY K Wu
Apr 29, 1998·Annual Review of Physiology·T L CreazzoM L Kirby
May 16, 2000·Circulation Research·D Srivastava
Jun 23, 2000·Developmental Biology·C M LiberatoreK E Yutzey
Nov 2, 2001·Nature Medicine·C J Hatcher, C T Basson
Feb 20, 2003·The Journal of Cell Biology·Josephine M EncisoJoseph A Madri
Mar 6, 2003·Reviews in Endocrine & Metabolic Disorders·Ulf J ErikssonParri Wentzel
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Gabriele E Ackermann, Barry H Paw
Oct 10, 2003·Nature·Adam F L HurlstoneHans Clevers
Sep 4, 2004·Circulation Research·Ehrin J Armstrong, Joyce Bischoff
Dec 4, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Daniël G M MolinAdriana C Gittenberger-De Groot
May 25, 2005·Diabetic Medicine : a Journal of the British Diabetic Association·G L NielsenA E Czeizel
Dec 22, 2006·Birth Defects Research. Part A, Clinical and Molecular Teratology·Pauline A M RoestAdriana C Gittenberger-De Groot
Jul 29, 2008·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Jörg Männer
Aug 5, 2008·American Journal of Obstetrics and Gynecology·Adolfo CorreaE Albert Reece
Mar 3, 2009·Genesis : the Journal of Genetics and Development·Francesca VitelliAntonio Baldini
Sep 1, 1994·Trends in Cardiovascular Medicine·D Y Stainier, M C Fishman

❮ Previous
Next ❯


Oct 23, 2010·General and Comparative Endocrinology·Agata JurczykPhilip diIorio
Dec 19, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Nadia TabassumDarren R Williams
Nov 30, 2012·Diabetes·Devon E Scott-DrechselMonica T Hinds
Apr 28, 2016·Upsala Journal of Medical Sciences·Ulf J Eriksson, Parri Wentzel
Nov 22, 2018·Obstetrics and Gynecology·UNKNOWN American College of Obstetricians and Gynecologists' Committee on Practice Bulletins—Obstetrics
Jul 4, 2019·Journal of Neurophysiology·Hannah EnnerfeltJessica Kennett Clark
Apr 1, 2021·British Journal of Pharmacology·George BowleyJovana Serbanovic-Canic
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Suneeta NarumanchiJere Paavola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.