Elevated HOXA1 expression correlates with accelerated tumor cell proliferation and poor prognosis in gastric cancer partly via cyclin D1

Journal of Experimental & Clinical Cancer Research : CR
Chenwei YuanChong-Zhi Zhou

Abstract

HOXA1 is a member of the Homeobox gene family, which encodes a group of highly conserved transcription factors that are important in embryonic development. However, it has been reported that HOXA1 exhibits oncogenic properties in many malignancies. This study focused on the expression and clinical significance of HOXA1 in gastric cancer (GC). To assess the mRNA and protein expression of HOXA1 and cyclin D1 in GC tissues, we utilized qRT-PCR and western blotting, respectively. The effects of HOXA1 on GC cell proliferation, migration, and invasion, as well as xenograft tumor formation and the cell cycle were investigated in our established stable HOXA1 knockdown GC cell lines. The protein expression of HOXA1 and cyclin D1 was examined by immunohistochemistry using GC tissue microarrays (TMA) to analyze their relationship on a histological level. The Kaplan-Meier method and cox proportional hazards model were used to analyze the relationship of HOXA1 and cyclin D1 expression with GC clinical outcomes. HOXA1 mRNA and protein expression were upregulated in GC tissues. Knockdown of HOXA1 in GC cells not only inhibited cell proliferation, migration, and invasion in vitro but also suppressed xenograft tumor formation in vivo. Moreover,...Continue Reading

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Citations

Feb 21, 2019·Experimental and Therapeutic Medicine·Jintao LiuXinyi Lu
Aug 22, 2017·Frontiers in Physiology·Xiao-Lei TangChun-Hui Yuan
Feb 11, 2021·Journal of Experimental & Clinical Cancer Research : CR·Chenlong Song, Chongzhi Zhou
Sep 8, 2021·Frontiers in Oncology·Magali BelpaireRené Rezsohazy
Oct 8, 2021·Cell Biology and Toxicology·U Sangeetha ShenoyRaghu Radhakrishnan

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Methods Mentioned

BETA
xenograft
transfection
PCR
protein assay
electrophoresis
flow cytometry
xenografts

Software Mentioned

SPSS

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