Elevated insulin receptor protein expression in experimentally induced colonic tumors

Cancer Letters
Gacheri A KiungaR P Bird

Abstract

Insulin is associated with augmented colon carcinogenesis in vivo, but the mechanism(s) of growth promotion is not known. This study investigates the expression profile of a key component of the insulin signaling pathway, the insulin receptor (IR), in colonic tumors in comparison to normal colonic mucosa and the effects of dietary lipids. Male F344 rats harboring preneoplastic lesions were randomly allocated to three high fat diet groups: beef tallow (HFB), corn oil (HFC), fish oil (HFF) or a low fat corn oil (LFC) diet for 16 weeks. Colonic tumors and mucosae were analyzed for IR protein by Western blot and immunohistochemical analyses. IR mRNA expression was also analyzed by semi-quantitative RT-PCR. Western blot analysis demonstrated that IR protein level was significantly greater (P < or =0.001) in tumors than in normal-appearing mucosae in each diet group, and varied (P < or =0.001) among the tumors in the order HFF>LFC>HFB>HFC. Immunohistochemical assessment of the tumors as compared to the normal mucosa confirmed the elevated expression of IR protein in the tumor cells compared to normal mucosae. Immunoreactivity was noted in the nuclear compartment. In normal colonic mucosae, IR protein levels were lower (P < or =0.001)...Continue Reading

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Citations

Dec 17, 2008·Nutrition Research Reviews·Maria Pufulete
Feb 5, 2016·Journal of Preventive Medicine and Public Health = Yebang Ŭihakhoe Chi·Hyeongtaek WooAesun Shin
Sep 11, 2014·Molecular Nutrition & Food Research·Yun-Ji KimMi-Kyung Sung
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Nov 30, 2018·Journal of Clinical Medicine·David NiederseerChristian Datz
Dec 18, 2020·BMC Medicine·Caroline J BullMarc J Gunter

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