Elevated intracellular Ca2+ acts through protein kinase C to regulate rabbit ileal NaCl absorption. Evidence for sequential control by Ca2+/calmodulin and protein kinase C

The Journal of Clinical Investigation
Mark DonowitzG W Sharp

Abstract

Calcium/calmodulin is involved in the regulation of basal rabbit ileal active Na and Cl absorption, but the mechanism by which elevated intracellular Ca2+ affects Na and Cl transport is unknown. To investigate the roles of the Ca2+/calmodulin and protein kinase C systems in ileal NaCl transport, two drugs, the isoquinolenesulfonamide, H-7, and the naphthalenesulfonamide, W13, were used in concentrations that conferred specificity in the antagonism of protein kinase C (60 microM H-7) and Ca2+/calmodulin (45 microM W13), respectively, as determined using phosphorylation assays in ileal villus cells. W13 but not H-7 stimulated basal active NaCl absorption. H-7 inhibited changes in Na and Cl absorption caused by maximal concentrations of Ca2+ ionophore A23187 and carbachol and serotonin, secretagogues that act by increasing cytosol Ca2+, while W13 had no effect. In contrast, neither H-7 nor W13 altered the change in NaCl transport caused by the cyclic nucleotides 8-Br-cAMP and 8-Br-cGMP. These data suggest that: (a) basal rabbit ileal NaCl absorption is regulated by the Ca2+/calmodulin complex and not by protein kinase C; (b) the effect of elevating intracellular Ca2+ to decrease NaCl absorption is mediated via protein kinase C but...Continue Reading

References

May 1, 1986·The New England Journal of Medicine·H Rasmussen
Jan 1, 1986·Annual Review of Physiology·M Donowitz, M J Welsh
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Aug 1, 1980·The Journal of Clinical Investigation·M DonowitzG Pike

❮ Previous
Next ❯

Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·A ItohY Okada
Nov 11, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·M W MuschE B Chang
Jul 1, 1994·Comparative Biochemistry and Physiology. Pharmacology, Toxicology and Endocrinology·C S HyunP I Karl
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·U SundaramJ W Dobbins
Feb 1, 1991·The Journal of Clinical Investigation·U SundaramJ W Dobbins
Nov 9, 2010·Annual Review of Physiology·Akira Kato, Michael F Romero
Jun 23, 2009·Annals of the New York Academy of Sciences·Nicholas C ZachosMark Donowitz
Oct 1, 2005·The Journal of Laboratory and Clinical Medicine·Seth C NarinsMohammad Z Abedin
Jul 7, 2007·Physiological Reviews·Mark Donowitz, Xuhang Li
Sep 5, 2003·American Journal of Physiology. Cell Physiology·Whaseon Lee-KwonMark Donowitz
May 25, 2013·American Journal of Physiology. Cell Physiology·Nicholas C ZachosMark Donowitz
Mar 11, 2000·American Journal of Physiology. Cell Physiology·J C OprinsJ A Groot
Sep 9, 1998·The American Journal of Physiology·R L McSwineE B Chang
Aug 13, 1999·The American Journal of Physiology·A P MorrisM K Estes
Jun 1, 1991·The American Journal of Physiology·E B ChangM C Rao
Mar 1, 1996·Microbiological Reviews·C L Sears, J B Kaper
Dec 15, 1989·Biochemical and Biophysical Research Communications·J A CohnW F King
Apr 1, 1996·Pediatric Clinics of North America·P M ShermanM B Cohen
Dec 1, 1996·Journal of Clinical Microbiology·A IsmailiP M Sherman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.