Elevated intracellular levels of cAMP induce olfactory ensheathing cells to express GAL-C and GFAP but not MBP

Glia
R Doucette, R Devon

Abstract

The primary olfactory pathway contains non-myelinating glial cells, called ensheathing cells, that exhibit a variety of phenotypes depending on their immediate environment. In vivo, these cells normally possess a mixture of astrocyte- and Schwann cell-specific phenotypic features. When co-cultured with dorsal root ganglion neurons, their phenotype can become more like that of a myelinating Schwann cell. The objective of this study was to determine whether ensheathing cells would express a myelinating phenotype in culture in the absence of neurons but in the presence of cAMP analogues that are known to induce the expression of myelin associated molecules in Schwann cell cultures. The ensheathing cell cultures were initiated using the nerve fiber layers of Theiler stage 23 rat olfactory bulb primordia and were fed for 1 day to 3 weeks with serum containing (1% or 10% FBS) or serum-free media to which was added different concentrations of dBcAMP (0.1 to 1 mM) or forskolin (10 microM). These cultures were double-labelled with a rabbit polyclonal antibody to S100 in combination with mouse anti-GAL-C (O1 and BRD1 hybridomas) or anti-MBP monoclonal antibodies. The remaining cultures were double-labeled with a rabbit polyclonal antibod...Continue Reading

References

Jul 11, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R B NorgrenR Brackenbury
Aug 28, 1992·Brain Research·R Devon, R Doucette
Jun 1, 1990·Journal of Neuroscience Research·K R BrundenJ F Poduslo
Oct 1, 1990·Journal of Neuroscience Research·K R Brunden, D T Brown
May 22, 1991·The Journal of Comparative Neurology·F Valverde, L Lopez-Mascaraque
Sep 1, 1991·Journal of Neurocytology·S MorrisonD Kirkham
Feb 1, 1986·The Journal of Cell Biology·M Dubois-DalcqR A Lazzarini
Jan 1, 1985·Developmental Neuroscience·D PleasureG Sobue
Dec 1, 1989·Neuron·E S MonukiG Lemke
Jul 22, 1989·The Journal of Comparative Neurology·R Doucette
Dec 1, 1987·Journal of Neurochemistry·R Bansal, S E Pfeiffer
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert
Feb 1, 1993·Developmental Biology·S C BarnettM Noble
Feb 15, 1993·Journal of Neuroscience Research·D W Raible, F A McMorris

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Citations

Jun 22, 2006·Cell and Tissue Research·Christiane KrudewigKonstantin Wewetzer
Jun 28, 2000·Progress in Neurobiology·A Mackay-Sima, M I Chuahb
May 16, 1997·Neuroscience Letters·A Bakardjiev
Jul 17, 1998·Brain Research Bulletin·A Ramón-Cueto, J Avila
Apr 5, 2003·Molecular and Cellular Neurosciences·Shaista HayatJon Robbins
Feb 7, 2009·Journal of Neurotrauma·Michael D KawajaRon Doucette
Jan 22, 2002·Journal of Computer Assisted Tomography·Robert I Henkin, Lucien M Levy
May 21, 2014·Experimental Neurology·Kasper C D Roet, Joost Verhaagen
Mar 6, 2003·The Anatomical Record. Part B, the New Anatomist·J G BoydR Doucette
May 12, 2007·The Journal of Comparative Neurology·Ali JahedMichael D Kawaja
Jun 26, 1998·Experimental Neurology·N LiuF J Roisen
May 13, 2017·Neurochemical Research·Karen L LankfordJeffery D Kocsis

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