Elevated messenger RNA expression and plasma protein levels of osteopontin and matrix metalloproteinase types 2 and 9 in patients with ascending aortic aneurysms

The Journal of Thoracic and Cardiovascular Surgery
Tuija HuuskoSakari Kakko

Abstract

Ascending aortic aneurysms result from a degenerative process in the aortic wall, characterized by the loss of smooth muscle cells and elastic fibers. We hypothesized that there would be changes in plasma protein and aortic tissue messenger RNA levels of osteopontin, matrix metalloproteinase type 2, matrix metalloproteinase type 9, and tissue inhibitor of matrix metalloproteinases type 1 in ascending aortic aneurysm samples. Plasma, aortic tissue, and aortic mRNA samples were collected from patients with an ascending aortic aneurysm or an abdominal aortic aneurysm and from control individuals. Plasma protein levels of osteopontin, matrix metalloproteinase (MMP) types 2 and 9, and tissue inhibitor of matrix metalloproteinases type 1 were determined by quantitative sandwich enzyme-linked immunosorbent assay. Aortic mRNA levels of these same proteins were analyzed with the quantitative real-time polymerase chain reaction (RT-PCR) method and protein levels from the aortic tissues were assayed by immunostaining. Quantitative RT-PCR results were estimated by the normalized expression method (ΔΔCt). Plasma protein levels were significantly elevated for osteopontin, MMP-2, and MMP-9 in the samples of ascending and abdominal aortic aneu...Continue Reading

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Citations

Aug 19, 2014·The British Journal of Surgery·P W StatherM J Bown
Jan 1, 2014·International Scholarly Research Notices·Konstantinos FilisGeorgios Zografos
Sep 24, 2016·Interactive Cardiovascular and Thoracic Surgery·Philipp MeffertFabian A Kari
Dec 6, 2017·General Thoracic and Cardiovascular Surgery·Ayman A SaeyeldinJohn A Elefteriades
Jul 4, 2015·Circulation Research·Christina L PapkeHiromi Yanagisawa
Oct 19, 2019·Journal of Clinical Medicine·Stasė GasiulėGiedrius Vilkaitis
Feb 19, 2014·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Yan fei LiuZheng Xu
Apr 28, 2017·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Brian J Malm, Mehran M Sadeghi
Jan 29, 2019·Mediators of Inflammation·Fábio Haach TéoMaria Heloisa Souza Lima Blotta

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