Elevated methylation of OPRM1 and OPRL1 genes in Alzheimer's disease

Molecular Medicine Reports
Chunshuang XuQinwen Wang

Abstract

Previous studies have suggested that increased opioid receptor κ1 (OPRK1) and opioid receptor δ1 (OPRD1) methylation levels are involved in Alzheimer's disease (AD). In the present study, the methylation levels of two opioid receptor genes, opioid receptor µ1 (OPRM1) and opioid related nociceptin receptor 1 (OPRL1), were analyzed for their association with AD. Gene methylation levels were measured using bisulfite pyrosequencing in DNA samples derived from blood samples of 51 AD patients and 63 controls. The results indicated that there were significantly elevated promoter methylation levels of OPRM1 and OPRL1 in AD (OPRM1: P=0.007; OPRL1: P=2.987x10‑6). Dual‑luciferase reporter gene assays demonstrated that the promoter fragments of these two genes were able to promote gene expression (OPRM1: Fold‑change=2.616, P=0.003; OPRL1: Fold change=11.395, P=0.007). In addition, receiver operating characteristic analyses further indicated that a methylation panel of four opioid receptor genes (area under the curve=0.848, sensitivity=0.723, and specificity=0.879) performed well in the prediction of AD. These results suggested that opioid receptor genes may be used as potential methylation biomarkers for the diagnosis of AD.

References

Jan 1, 1979·Annual Review of Neuroscience·S H Snyder, S R Childers
Dec 31, 1993·Brain Research·J BargC J Coscia
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·C Gavériaux-RuffB L Kieffer
Apr 28, 2006·International Journal of Adolescent Medicine and Health·Alexis H Conason, Leo Sher
Aug 5, 2010·Clinical Medicine & Research·Nader GhebraniousCatherine A McCarty
Mar 30, 2011·Pharmacological Reviews·Christopher W CunninghamThomas E Prisinzano
Jun 3, 2011·World Psychiatry : Official Journal of the World Psychiatric Association (WPA)·UNKNOWN International Advisory Group for the Revision of ICD-10 Mental and Behavioural Disorders
Apr 25, 2012·Neuromolecular Medicine·Zhiyou Cai, Anna Ratka
May 2, 2012·Human Genetics·Orna LevranMary Jeanne Kreek
Nov 13, 2012·Archives of Medical Research·María Elisa Alonso VilatelaPetra Yescas-Gómez
Jan 2, 2013·Frontiers in Bioscience (Elite Edition)·Irene PiaceriSandro Sorbi
Mar 30, 2013·AJNR. American Journal of Neuroradiology·A T RaoL M Levy
Oct 12, 2013·Folia Neuropathologica·R A Armstrong
Jun 26, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chi T VietBrian L Schmidt
Aug 26, 2014·CNS Neuroscience & Therapeutics·Yan WangHong-Zhuan Chen
Nov 18, 2014·Frontiers in Neuroscience·Paul A AdlardGary F Egan
Aug 25, 2015·Neuroscience Letters·Huihui JiQinwen Wang
Nov 5, 2015·PloS One·Yuan SunJ David Clark

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Citations

Nov 13, 2020·Frontiers in Neurology·Xuewen XiaoBin Jiao

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Methods Mentioned

BETA
transfections
Assay

Software Mentioned

SPSS

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