Abstract
Regulatory T cells (Tregs) have been related to acute rejection as have the cytotoxic T cells, their immunological counterpart. High expression of cytotoxic markers has been related to acute rejection incidents following both kidney and intestine transplantation, while the correlation between FoxP3 expression and acute rejection is still being debated. Some studies have been performed on blood samples from lung-transplanted patients, while others have investigated the local immune response in the lungs by analysing broncho-alveolar-lavage (BAL) fluids or biopsies. Biopsies are considered the gold standard in diagnosis of acute rejection. The aim was to measure the expression of both Treg (FoxP3, CD25 and CTLA-4) and cytotoxic (granzyme B, granulysin, and perforin) markers in BAL and blood samples from lung-transplanted patients to investigate the possible relation of expression and acute rejection incidents in order to develop a non-invasive diagnostic method for acute rejection. 24 lung-transplanted patients were included in this 6-month cross-section study. BAL and blood samples were analysed for FoxP3, CD25, CTLA-4, granzyme B, granulysin, perforin, CD4 and CD8 mRNA by QRT-PCR with glyceraldehyde 3-phosphate dehydrogenase (G...Continue Reading
References
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 18, 2004·Transplant Immunology·Ruili ShiT Mohanakumar
Jun 30, 2004·Transplantation·Katja KotschPetra Reinke
Apr 13, 2005·Nephron. Clinical Practice·Gyu-Tae ShinHeungsoo Kim
Sep 9, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Roy Calne
Oct 19, 2005·Cellular and Molecular Life Sciences : CMLS·K SchepersT N M Schumacher
Dec 2, 2005·The New England Journal of Medicine·Thangamani MuthukumarManikkam Suthanthiran
Jan 3, 2006·Transplantation Proceedings·B CortiA D'Errico-Grigioni
Feb 1, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Ahmet DemirkiranLuc J W van der Laan
May 17, 2006·Transplant Immunology·Agata KosmaczewskaMarian Klinger
Aug 16, 2006·Transplantation Proceedings·B CortiA D Grigioni
Aug 22, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Maria YannarakiChristophe Ferrand
Oct 24, 2006·Human Immunology·Shuiping JiangJie-Fu Huang
Feb 9, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·F VeroneseR B Colvin
Jun 23, 2007·Transplant Immunology·G VladN Suciu-Foca
Dec 1, 2007·Transplant Immunology·I Esmé DijkeCarla C Baan
Dec 22, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Susan StewartSamuel A Yousem
Mar 19, 2008·Transplantation·Christopher M BurtonClaus B Andersen
Apr 15, 2008·Transplantation·Aric L GregsonOtto O Yang
Jun 21, 2008·Clinical Transplantation·Emmanuel BoleslawskiFilomena Conti
Jul 1, 2008·Transplantation Proceedings·I E DijkeC C Baan
Jul 22, 2008·Human Immunology·I Esmé DijkeCarla C Baan
Mar 17, 2009·Scandinavian Journal of Immunology·A ØzbayJ Carstens
Citations
Nov 16, 2013·PloS One·David San SegundoMarcos López-Hoyos
May 8, 2014·Transplant Immunology·Dorrit KrustrupClaus B Andersen
Jul 19, 2011·Blood Reviews·Shahinaz M Gadalla, Sharon A Savage
Sep 22, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R TaubertE Jaeckel
Nov 8, 2014·European Journal of Immunology·Anne M PesenackerMegan K Levings
Aug 24, 2013·Transplant Immunology·Dorrit KrustrupClaus B Andersen
May 24, 2011·PloS One·Maud RacapéSophie Brouard
Nov 26, 2015·Science Advances·Andrew T SageShana O Kelley
Jan 9, 2019·Connective Tissue Research·Ming-Xi LiuDa-Hui Sun
May 29, 2018·Respiratory Research·Liran LevyTereza Martinu