Elevated plasma cell membrane glycoprotein levels and diminished insulin receptor autophosphorylation in obese, insulin-resistant rhesus monkeys

Metabolism: Clinical and Experimental
Celia PenderJ Youngren

Abstract

In obese humans, insulin resistance is accompanied by elevated levels of plasma cell membrane glycoprotein (PC-1) and decreased insulin receptor (IR) tyrosine kinase activity in skeletal muscle. PC-1 overexpression inhibits IR tyrosine kinase and possibly other downstream signaling events. The rhesus monkey in captivity is susceptible to obesity with concomitant insulin resistance. In the present study we analyzed obese (n = 10, 29.4% +/- 1.2% body fat) and non-obese (n = 12, 19.4% +/- 1.9% body fat) rhesus monkeys. Glucose clearance during an euglycemic hyperinsulinemic (400 mU/m(2) body surface area/min) clamp was lower for the obese group (non-obese, 9.7 +/- 0.9; obese, 3.2 +/- 0.7 mg/kg fat-free mass [FFM]/min; P <.01). We performed vastus lateralis muscle biopsies prior to and during the clamp. We measured PC-1 levels in these muscle samples to determine whether PC-1 content is elevated in this primate model of insulin resistance. PC-1 levels were determined by assay of phosphodiesterase activity and specific PC-1 enzyme-linked immunosorbent assay (ELISA). In the obese group, both PC-1 content and activity were 2-fold higher than in the non-obese group (P <.05). In order to investigate the ability of insulin to stimulate I...Continue Reading

Citations

Feb 22, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·N TorresA L Benabid
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Philipp EllerJosef R Patsch
Nov 10, 2005·American Journal of Physiology. Endocrinology and Metabolism·Betty A MadduxIra D Goldfine
Aug 30, 2005·Trends in Biochemical Sciences·Cristiana StefanMathieu Bollen

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