Elevated Plasma Levels of Cell-Free DNA During Liver Transplantation Are Associated With Activation of Coagulation

Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Fien A von MeijenfeldtT Lisman

Abstract

Patients undergoing liver transplantation have complex changes in their hemostatic system, and the net effect of these changes appears to be a "rebalanced" hemostatic profile. Recently, a process called NETosis in which a neutrophil expels DNA and proteins that form a weblike structure, has been described as a mechanism of pathogen entrapment. Increasing evidence suggests a pivotal role for neutrophil extracellular traps (NETs) and their main component, cell-free DNA (cfDNA), in activation of coagulation. Because liver transplantation is associated with substantial (hepatocyte) cell death and intrahepatic neutrophil accumulation, NETs might play an important role in the hemostatic balance during liver transplantation. Here, we determined markers for NETs in the plasma of patients undergoing a liver transplantation and examined their association with activation of coagulation. Markers for NETs and markers for activation of coagulation were determined in serial plasma samples taken from patients undergoing a liver transplantation (n = 21) and compared with plasma levels in healthy controls. We found perioperative increases of markers for NETs with levels of cfDNA and nucleosomes that peaked after reperfusion and myeloperoxidase (...Continue Reading

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Citations

Oct 30, 2018·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Danielle H Moore, Neeral L Shah
Oct 20, 2018·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Elzbieta Kolaczkowska, Craig N Jenne
Jun 30, 2019·Journal of Thrombosis and Haemostasis : JTH·Dafna J Groeneveld, James P Luyendyk
Nov 30, 2019·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Osamu YoshinoSu Kah Goh
Sep 10, 2020·Seminars in Thrombosis and Hemostasis·Fien A von Meijenfeldt, Craig N Jenne
May 9, 2019·Seminars in Cardiothoracic and Vascular Anesthesia·Natalie K SmithTetsuro Sakai
Sep 16, 2020·Cancers·Michal MegoPeter Celec
Nov 22, 2020·Journal of Thrombosis and Haemostasis : JTH·Fien A von MeijenfeldtWilliam Bernal
Dec 15, 2020·World Journal of Transplantation·Tess McClureAdam G Testro

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Methods Mentioned

BETA
ELISA
DNA assay
ELISAs
biopsies
biopsy

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