Elevated substance P and accelerated cartilage degradation in rabbit knees injected with interleukin-1 and tumor necrosis factor

Arthritis and Rheumatism
E M O'ByrneA Y Jeng

Abstract

Cytokines, interleukin-1 (IL-1), tumor necrosis factor alpha, and the neurotransmitter, substance P, have been implicated in the pathogenesis of arthritis because they stimulate synovial cells to secrete prostaglandin E2 and collagenase in vitro. We investigated in vivo changes in intraarticular substance P and the degradation of cartilage proteoglycan in response to intraarticular cytokine injections in rabbits. Twenty-four hours after a single injection of 10 ng, 30 ng, or 100 ng of recombinant human IL-1 alpha (rHuIL-1 alpha) per joint, the mean +/- SEM levels of substance P detected in the cell-free joint lavage fluid were 250 +/- 67 fmoles, 480 +/- 60 fmoles, and 530 +/- 130 fmoles (n = 4-5), respectively. The level of substance P in the contralateral knees injected with diluent was 58 +/- 8 fmoles (n = 12). The level of substance P had increased by 2 hours after IL-1 injection and remained elevated in the joint 48 hours after injection. Cytokine-induced proteoglycan depletion was also time- and dose-dependent. Proteoglycan concentrations in articular cartilage dissected from the weight-bearing condyles were calculated as the ratio of sulfated glycosaminoglycan measured using 1,9-dimethylmethylene blue: hydroxyproline. Aft...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C A DinarelloS M Wolff
Mar 1, 1990·Analytical Biochemistry·A Y JengJ P Gilligan
May 1, 1989·Arthritis and Rheumatism·E R PettipherA Ratcliffe
Sep 1, 1988·British Journal of Pharmacology·E R PettipherG A Higgs
Sep 7, 1987·Life Sciences·J P Pujol, G Loyau
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E R PettipherB Henderson
Dec 1, 1986·Agents and Actions·E R PettipherB Henderson
Jul 1, 1987·Annals of the Rheumatic Diseases·J T DingleD R Bard
Jun 1, 1986·The Journal of Clinical Investigation·C A DinarelloR C Reuben
Dec 1, 1973·Scandinavian Journal of Clinical and Laboratory Investigation·L Tougaard
Apr 30, 1981·Biochemical and Biophysical Research Communications·W A MarascoE L Becker
Jan 1, 1982·Pharmacology·R Couture, D Regoli
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Jan 1, 1984·Connective Tissue Research·J T Dingle
May 1, 1982·Arthritis and Rheumatism·D M Mitchell, J F Fries

❮ Previous
Next ❯

Citations

Dec 31, 1998·Arthritis and Rheumatism·B BresnihanP Musikic
Jul 1, 1993·Journal of Magnetic Resonance Imaging : JMRI·L A JelicksR K Gupta
Aug 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·R L GoldbergS Spirito
Mar 28, 1996·European Journal of Pharmacology·M MalcangioM Perretti
Mar 1, 1995·Bioorganic & Medicinal Chemistry·S W WrightM A Pratta
Jun 1, 1996·Bioorganic & Medicinal Chemistry·S W WrightE C Arner
Nov 23, 2000·British Journal of Pharmacology·S CuzzocreaA P Caputi
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M Freidin, J A Kessler
Jun 1, 1994·Annals of the Rheumatic Diseases·K P Pritzker
Sep 21, 2001·Current Rheumatology Reports·U Fearon, D J Veale
Mar 10, 2001·Journal of Neuroimmunology·I Marriott, K L Bost
Mar 1, 1994·British Journal of Pharmacology·M K Herbert, P Holzer
Jan 5, 2001·Expert Opinion on Investigational Drugs·S Brunelleschi
Jan 1, 1994·Scandinavian Journal of Rheumatology·P KortekangasT J Nevalainen
Sep 3, 2011·International Journal of Oral and Maxillofacial Surgery·N GülerB Cam
Jul 8, 2011·PM & R : the Journal of Injury, Function, and Rehabilitation·Michael B Mueller, Rocky S Tuan
Sep 22, 2009·International Journal of Experimental Pathology·Nidhi Sofat
Jul 11, 2006·International Journal of Oral and Maxillofacial Surgery·G CledesP Carpentier
Jun 1, 1992·Baillière's Clinical Rheumatology·J M Dayer, H Fenner
Oct 30, 2014·Journal of Cellular and Molecular Medicine·M MazorH Toumi
Dec 24, 2013·Journal of Biomechanics·Stephanie GrenierPeter A Torzilli
Jul 17, 2016·Current Rheumatology Reports·Antonia RuJia SunIndira Prasadam
Jan 1, 1995·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C L HaskinI L Cameron
May 7, 2003·The Journal of Biological Chemistry·Mátyás CzipriTibor T Glant
Aug 31, 2004·Journal of Cellular Physiology·Terence M O'ConnorFergus Shanahan
Jul 1, 1995·Canadian Journal of Physiology and Pharmacology·J E KrauseK E McCarson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.