Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer

Archivum Immunologiae Et Therapiae Experimentalis
Joanna Domagała-KulawikRyszarda Chazan

Abstract

Transforming growth factor (TGF)-beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. The aim of this study was to evaluate TGF-beta1 levels and lymphocyte subsets in the broncholaveolar lavage fluid (BALF) of patients with primary lung cancer and to analyze the interdependence of these parameters. BALF samples were collected from 38 patients with primary lung cancer prior to treatment and from 23 healthy volunteers. Concentrations of TGF-beta1 were measured in two independent lots of samples using a commercially available sandwich ELISA kit after concentration of the supernatants. Differential cell counts in the BALF were performed on slides stained with the May Grünwald Giemsa method. Flow cytometry with monoclonal antibodies was applied for lymphocyte phenotyping. A higher level of TGF-beta1 in the BALF of patients compared with the healthy subjects was observed in both lots of samples (3.23+/-2.96 pg/ml vs. 1.05+/-0.95 pg/ml, p<0.05, and 16.1+/-19.3 pg/ml vs. 10.1+/-11.1 pg/m,, respectively, difference not significant). There was significant positive correlation of the TGF-beta1 level with the proportion of lymphocytes and negative correlation with both the proportion of macr...Continue Reading

Citations

Apr 29, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Randeep S JawaHeinz Baumann
May 8, 2010·Journal of Cancer Research and Clinical Oncology·Ana L TeixeiraRui Medeiros
May 30, 2014·Journal of Immunology Research·Min-Chao DuanJin-Ru Wei
Mar 21, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ye-Sheng WeiLin Zhang
Jan 9, 2017·Clinical Oncology : a Journal of the Royal College of Radiologists·R MarshallT Illidge
Jan 28, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Sunhong HuMang Xiao
Dec 12, 2017·Breathe·Joanna Domagala-Kulawik, Agata Raniszewska
Mar 29, 2019·Journal of Cellular Biochemistry·Xiaoming ZhangChanggang Sun
Jun 4, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Chao CaoZai-Chun Deng
Dec 24, 2019·Expert Review of Respiratory Medicine·Joanna Domagala-Kulawik
Jul 26, 2011·Journal of Clinical Laboratory Analysis·Ana E González-SantiagoCarmen M Gurrola-Díaz
Dec 13, 2019·Pulmonary Medicine·Rani SinghSidhu P Gangadharan
Nov 28, 2015·Respirology : Official Journal of the Asian Pacific Society of Respirology·Yingwei ZhangHourong Cai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.