PMID: 6169431Oct 1, 1981Paper

Elevated transcriptional complexity and decrease in enzymatic DNA methylation in cells treated with L-ethionine

Cancer Research
T L Boehm, D Drahovsky

Abstract

This paper analyzes the extent of enzymatic methylation of DNA in P815 cells cultured for several cell cycles in the presence of 1 mM L-ethionine by use of the bacterial restriction enzymes HpaII, MspI, HhaI, HaeII, and AvaI. The results obtained suggest that the extent of methylation of DNA is decreased during L-ethionine treatment and that the degree of this inhibition varies among different methylation sites. The nucleotide sequence complexity of nuclear RNA in P815 cells was analyzed by excess hybridization with tracer amounts of nick-translated nonrepetitive P815 [3H]DNA sequences. About 6.2% of the nonrepetitive sequences form hybrids with nuclear RNA of controls cells, whereas 8.8% are complementary to nuclear RNA from L-ethionine-treated cells. Thus, the decrease in enzymatic DNA methylation correlates with the presence of new transcriptional products suggesting that this modification process inversely correlates with the initiation of transcription.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.