Elevating vitamin C content via overexpression of myo -inositol oxygenase and l-gulono-1,4-lactone oxidase in Arabidopsis leads to enhanced biomass and tolerance to abiotic stresses

In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association
Katherine A LiskoArgelia Lorence

Abstract

l-Ascorbic acid (vitamin C) is an abundant metabolite in plant cells and tissues. Ascorbate functions as an antioxidant, as an enzyme cofactor, and plays essential roles in multiple physiological processes including photosynthesis, photoprotection, control of cell cycle and cell elongation, and modulation of flowering time, gene regulation, and senescence. The importance of this key molecule in regulating whole plant morphology, cell structure, and plant development has been clearly established via characterization of low vitamin C mutants of Arabidopsis, potato, tobacco, tomato, and rice. However, the consequences of elevating ascorbate content in plant growth and development are poorly understood. Here we demonstrate that Arabidopsis lines over-expressing a myo-inositol oxygenase or an l-gulono-1,4-lactone oxidase, containing elevated ascorbate, display enhanced growth and biomass accumulation of both aerial and root tissues. To our knowledge this is the first study demonstrating such a marked positive effect in plant growth in lines engineered to contain elevated vitamin C content. In addition, we present evidence showing that these lines are tolerant to a wide range of abiotic stresses including salt, cold, and heat. Total ...Continue Reading

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Citations

Mar 25, 2020·Critical Reviews in Biotechnology·Hasthi RamTilak Raj Sharma
Nov 2, 2019·Antioxidants·Costantino PaciollaMaria Concetta de Pinto
Feb 2, 2017·BMC Plant Biology·Eric R SchultzRobert J Ferl
Jan 15, 2015·Annals of the New York Academy of Sciences·Javier A Miret, Sergi Munné-Bosch
Mar 11, 2020·International Journal of Molecular Sciences·Ronan C BroadAlexander A T Johnson
Apr 18, 2020·Plant Physiology and Biochemistry : PPB·Lucia M Acosta-GamboaLorence Argelia
Sep 15, 2021·The Plant Journal : for Cell and Molecular Biology·Pavel I Kerchev, Frank Van Breusegem

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