Elevation of androgen receptor promotes prostate cancer metastasis by induction of epithelial-mesenchymal transition and reduction of KAT5

Cancer Science
Ching-Yu LinChih-Pin Chuu

Abstract

Androgen receptor (AR), an androgen-activated transcription factor, belongs to the nuclear receptor superfamily. AR plays an important role in the development and progression of prostate cancer (PCa). However, the role of AR in PCa metastasis is not fully understood. To investigate the role of AR in PCa metastasis, we examined AR expression level in primary and metastatic PCa by analyzing gene array data of 378 primary prostate tumors and 120 metastatic prostate tumors from Oncomine, as well as carrying out immunohistochemical (IHC) staining of 56 prostate cancer samples. Expression of mRNA and protein of AR as well as its target gene prostate-specific antigen (PSA) was much higher in metastatic prostate tumors than in primary prostate tumors. Knockdown of AR with siRNA or treating with anti-androgen Casodex reduced migration and invasion ability of C4-2B PCa cells. Knockdown of AR increased protein expression of E-cadherin and AR coregulator KAT5 but reduced expression of epithelial-mesenchymal transition (EMT) marker proteins Slug, Snail, MMP-2, vimentin, and β-catenin. Knockdown of KAT5 increased migration of C4-2B cells, whereas overexpression of KAT5 suppressed cell migration. KAT5 knockdown rescues the suppressive effect ...Continue Reading

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Citations

Mar 28, 2019·Frontiers in Oncology·Katia A CheaitoWassim Abou-Kheir
May 7, 2020·Cancer Metastasis Reviews·Yannasittha Jiramongkol, Eric W-F Lam
Sep 26, 2020·Genomics·Irengbam Rocky MangangchaR K Brojen Singh
Oct 17, 2020·NPJ Breast Cancer·Anna R MichmerhuizenCorey W Speers
Feb 5, 2022·Free Radical Research·Sun Young Kim

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Datasets Mentioned

BETA
GE58203
GDS
2545

Methods Mentioned

BETA
biopsy
PCR
transfection
gene array
acetylation

Software Mentioned

TissueScan
Casodex
SurvExpress
Oncomine

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