Elevation of Autoantibody in Patients with Ischemic Stroke

Neurologia Medico-chirurgica
Yoichi YoshidaYasuo Iwadate

Abstract

Recent clinical research has revealed a significant correlation between atherosclerosis, one of the primary etiologies of ischemic stroke, and the immune system. Assuming that "disease-specific autoantibodies are produced in the sera of patients with ischemic stroke," we investigated multiple arteriosclerosis-related antibodies using the serological identification of antigens by recombinant cDNA expression cloning (SEREX), an established method for identifying antigenic proteins. We either screened a human aortic endothelial cell cDNA library or conducted protein array screening using the sera from patients with ischemic stroke, such as carotid artery stenosis or transient ischemic attack (TIA). Next, we measured serum antibody levels using amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) in patient/healthy donor (HD) cohorts and identified several antigens, the antibody levels of which were significantly higher in patients with ischemic stroke than in HDs. This review introduced the method of identifying antigens by the SEREX and protein microarray and summarized antigenic proteins. In particular, it focused on anti-replication protein A2 antibody and anti-programmed cell death 11 antibo...Continue Reading

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Citations

Jul 10, 2019·Neurologia Medico-chirurgica·Takeshi MikamiNobuhiro Mikuni
Apr 2, 2021·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Kazuo SugimotoSatoshi Kuwabara
Apr 23, 2021·BMC Neurology·Hime SuzukiNobuhiro Mikuni
Nov 7, 2021·Scientific Reports·Masaaki KubotaYasuo Iwadate

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Methods Mentioned

BETA
assay-linked immunosorbent assay
AlphaLISA
enzyme-linked immunosorbent assay

Software Mentioned

AlphaLISA

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