Elevation of [Ca2+]i of renal proximal tubular cells and down-regulation of mRNA of PTH-PTHrP, V1a and AT1 receptors in kidney of diabetic rats

Kidney International
W MarcinkowskiS G Massry

Abstract

An elevation in intracellular calcium ([Ca2+]i) in rats with chronic renal failure and elevated blood levels of PTH is associated with down-regulation of the mRNA of many proteins. Similarly, in phosphate depleted animals that have normal renal function and low blood levels of PTH, [Ca2+]i is elevated and the mRNA of PTH-PTHrP receptor is down-regulated. The effect of elevation in [Ca2+]i on molecular machinery of many proteins may represent a generalized phenomenon. Diabetes mellitus may also be associated with a rise in [Ca2+]i and therefore down-regulation of the mRNA of proteins may also occur. The present study examined the effect of streptozotocin-induced diabetes mellitus in rats on the [Ca2+]i of the renal proximal tubular cells and on their mRNAs of the PTH-PTHrP, V1a and AT1 receptors. The basal levels of [Ca2+]i of these cells increased significantly (P < 0.01) after one day of diabetes and remained elevated thereafter. There was a significant (r = 0.67, P < 0.01) direct correlation between the [Ca2+]i of the cells and blood levels of glucose up to 350 mg/dl, and the value of [Ca2+]i plateaued with higher concentrations of glucose. Three days of amlodipine therapy prevented and reversed the elevated levels of [Ca2+]i...Continue Reading

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Citations

Jun 26, 2001·Diabetes/metabolism Research and Reviews·W H Taylor, A A Khaleeli
Aug 28, 2003·Clinical and Experimental Pharmacology & Physiology·Ho Jae HanJang Hern Lee
Aug 4, 2007·Kidney International. Supplement·Y J LeeH J Han
Jul 26, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·P Esbrit, J Egido
Jan 15, 2002·American Journal of Physiology. Renal Physiology·Soo Hyun Park, Ho Jae Han
Mar 1, 2013·Bone Research·Francisco J A de Paula, Clifford J Rosen

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