Elevation of endogenous nucleophiles in rat lung by cysteine and glutathione esters in vitro

Biochemical Pharmacology
M HobbsD G Upshall

Abstract

In this study, we have compared the uptake of L-cysteine (L-CySH), D-cysteine (D-CySH), L-cysteine isopropyl ester (L-CIPE) and D-cysteine isopropyl ester (D-CIPE) in rat lung slices and tracheal sections and determined the effectiveness of glutathione (GSH), GSH isopropyl monoester, GSH isopropyl diester, gamma-glutamylcysteine (gamma-glu-cys) isopropyl monoester and gamma-glu-cys isopropyl diester to elevate and prolong intracellular GSH concentrations in rat lung slices. Lung slices were incubated with 1.0 mM of thiol and the concentrations determined intracellularly and extracellularly with time. Slices incubated with GSH, GSH isopropyl diester and gamma-glu-cys isopropyl diester had cellular GSH concentrations increased by up to 60%, 95% and 58%, respectively, whereas GSH isopropyl monoester and gamma-glu-cys isopropyl monoester did not increase the intracellular GSH concentration. Extracellularly, the GSH concentration had decreased by 15%, GSH isopropyl diester by 27%, gamma-glu-cys isopropyl diester by 66% and both isopropyl monoesters by over 90% at 120 min. Lung slices and tracheal sections incubated with L- or D-CySH at 37 degrees had increased cellular concentrations of L- and D-CySH which ranged between 0.88-1.25 n...Continue Reading

References

Dec 11, 1991·Biochemical Pharmacology·A F LaileyD G Upshall
Apr 15, 1991·The Biochemical Journal·P K Thornalley
Nov 15, 1989·Analytical Biochemistry·M E Anderson, A Meister
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L H LashD P Jones
Jun 1, 1985·Archives of Biochemistry and Biophysics·M E AndersonA Meister
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S Segal, J C Crawhall
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V P WellnerA Meister
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R N Puri, A Meister
Nov 1, 1994·Human & Experimental Toxicology·P E Wilde, D G Upshall
Nov 1, 1994·Human & Experimental Toxicology·A F Lailey, D G Upshall
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E J LevyA Meister
Jan 7, 1993·Biochemical Pharmacology·N W McCrackenF M Williams

❮ Previous
Next ❯

Citations

Mar 15, 2019·Journal of Research on Adolescence : the Official Journal of the Society for Research on Adolescence·Elizabeth J SusmanNilam Ram
Dec 22, 1999·The American Journal of Physiology·I Rahman, W MacNee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.