Elevation of intracellular calcium levels in outer hair cells by trimethyltin

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Y Liu, L D Fechter

Abstract

Outer hair cells are thought to modulate sensitivity of the peripheral auditory system to sound and they are frequently targets of injury by ototoxicants. These cells show an unusual motility that is thought to be critical to their physiological role and it is known that persistent contraction can be stimulated by an enhancement in intracellular calcium concentration ([Ca(2+)]i). Trimethyltin (TMT) disrupts functioning in the outer hair cell in vivo and produces a persistent contraction of these cells in vitro. Experiments were designed to determine whether TMT can alter [Ca(2+)]i levels in isolated outer hair cells maintained in primary culture and to determine the source of such an increase. Parallel positive and negative controls were included to ensure that cell depolarization using K(+) did produce the expected enhancement of [Ca(2+)]i and that exogenous glutamate stimulation produced no shift in [Ca(2+)]i. Outer hair cells do not have glutamate receptors. The [Ca(2+)]i of isolated outer hair cells from guinea pigs were monitored dynamically using the calcium-sensitive fluorescent dye, Fura 2, after application of TMT (30 muM(-1) mm), KCl (70 mm) or glutamate (100 muM). Cells exposed to TMT show a slow but persistent [Ca(2...Continue Reading

References

Mar 1, 1978·The Journal of Physiology·P F Baker, P A McNaughton
Oct 1, 1978·The Journal of General Physiology·J Requena
Dec 11, 1992·Toxicology Letters·S OrreniusP Nicotera
May 11, 1992·Annals of the New York Academy of Sciences·P Nicotera, S Orrenius
Apr 15, 1991·Neuroscience Letters·T NakagawaT Uemura
Jul 11, 1991·Toxicology and Applied Pharmacology·W J ClericiL D Fechter
Jan 1, 1990·Journal of Toxicology and Environmental Health·P R YallapragadaD Desaiah
Feb 1, 1990·Cell Calcium·M W RoeB Herman
Aug 1, 1990·Toxicology and Applied Pharmacology·K M CroftonR Janssen
Aug 1, 1990·Toxicology and Applied Pharmacology·L D Fechter, L Carlisle
Jan 2, 1987·Science·R J Miller
Mar 30, 1987·Toxicology and Applied Pharmacology·H Komulainen, S C Bondy
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Jan 1, 1986·Hearing Research·L D FechterA L Nuttall
Jan 1, 1986·Toxicology and Applied Pharmacology·J S Young, L D Fechter
Jan 1, 1980·International Review of Cytology·A Godfraind-De Becker, T Godfraind
Apr 1, 1984·The Biochemical Journal·R H AshleyR Marchbanks
Jul 1, 1984·Physiological Reviews·H Rasmussen, P Q Barrett
Jun 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W D Atchison, M F Hare
Mar 1, 1995·Acta Oto-laryngologica·C MorgensternW H Zangemeister
May 1, 1995·Neurotoxicology and Teratology·Y Liu, L D Fechter
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G WalkerP G Gillespie
Jun 1, 1993·Toxicology and Applied Pharmacology·W J ClericiL D Fechter
Jul 1, 2006·The AAPS Journal·Evan L RiddleGlen R Hanson

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