Eleven microsatellites in an emerging invader, Phytolacca americana (Phytolaccaceae), from its native and introduced ranges

Applications in Plant Sciences
Kerin E BentleyRodney Mauricio

Abstract

To facilitate population genetic analyses, microsatellite markers were developed for pokeweed (Phytolacca americana), a large, weedy, perennial herb native to eastern North America that is emerging as a significant invasive species in China. We mined 1,100,538 Illumina MiSeq reads from genomic DNA for microsatellites and identified 58 primer pairs. We screened these primers for polymorphism in two native and two invasive populations. We identified 11 loci that amplified consistently. The number of alleles per locus ranged from two to six, and observed heterozygosity ranged from 0.00 to 1.00. All loci were largely monomorphic within populations but different among populations. The primers were of very limited use in the congener P. acinosa. These loci will provide a valuable resource to study the population genetics and invasion history of P. americana.

Citations

Dec 14, 2019·Applications in Plant Sciences·Mark A Chapman
Nov 8, 2017·Applications in Plant Sciences·Yueqin ChengHongwei Wang

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Methods Mentioned

BETA
PCR

Software Mentioned

CHECKER
GENEPOP
GenAlEx
MsatAllele
PAL Finder
GeneMarker
MICRO

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