ELF1-activated FOXD3-AS1 promotes the migration, invasion and EMT of osteosarcoma cells via sponging miR-296-5p to upregulate ZCCHC3

Journal of Bone Oncology
Lei Wang

Abstract

Osteosarcoma (OS) is a malignant carcinoma often occurring in adolescents. The critical function of long non-coding RNAs (lncRNAs) in cancer arouses increasing attention. Nevertheless, the specific function of FOXD3 Antisense RNA 1 (FOXD3-AS1) in OS has not been understood yet. In this research, FOXD3-AS1 showed strengthened level in OS specimens and cell lines, and its deficiency restrained cell migration, invasion and epithelial-to-mesenchymal transition (EMT) in OS. Then, we confirmed the interaction of FOXD3-AS1 with microRNA-296-5p (miR-296-5p) and that miR-296-5p overexpression blocked OS cell migration, invasion and EMT. Besides, miR-296-5p targeted zinc finger CCHC-type containing 3 (ZCCHC3), and FOXD3-AS1 released ZCCHC3 via sequestering miR-296-5p. Moreover, rescue assays delineated that ZCCHC3 upregulation neutralized the inhibitory effect of FOXD3-AS1 depletion on in vitro behaviors and in vivo tumorigenesis in OS. In addition, E74 like ETS transcription factor 1 (ELF1) stimulated FOXD3-AS1 transcription, and ELF1 silence-suppressed malignant phenotypes of OS cells were offset by FOXD3-AS1 upregulation. Overall, present work elucidated that ELF1-activated FOXD3-AS1 aggravated cell migration, invasion and EMT in OS v...Continue Reading

References

Feb 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stefan S BielackKurt Winkler
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Sep 20, 2011·Molecular Cell·Kevin C Wang, Howard Y Chang
Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Jun 14, 2014·Advances in Experimental Medicine and Biology·Madonna M McManusDennis P M Hughes
Aug 13, 2015·Journal of Translational Medicine·Danielle MaiaAndre Luiz Vettore
Jun 29, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Duo ZhangYang Jin
Dec 9, 2017·Biochemical and Biophysical Research Communications·Qingli Kong, Min Qiu
Dec 15, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoli WuLingyan Shi
Feb 22, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qingchun LuTao Ren
Nov 30, 2018·Journal of Experimental & Clinical Cancer Research : CR·Dong-Min ShiWei-Zhong Wu
Jan 3, 2019·Pathology, Research and Practice·Longlong ZhengXiaozhong Zhou
Mar 26, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xueli WangLei Wang
Aug 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Chen QiJiang Jianshuai
Aug 16, 2019·World Journal of Gastroenterology : WJG·Wei WangQing-Chang Li

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Citations

May 1, 2021·International Journal of Molecular Sciences·Francesca BaldiniAldo Pagano
Jul 2, 2021·Experimental Biology and Medicine·Zhichang PanYixin Shen

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Methods Mentioned

BETA
transfection
Protein Assay
PCR
immunoprecipitation
RIP
transfections
xenografts
ChIP

Software Mentioned

JASPAR
RNA22
GraphPad Prism
PITA
PicTar
GraphPad
FusionCapt Advance Fx5
microT
TargetScan

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