ELF3 is an antagonist of oncogenic-signalling-induced expression of EMT-TF ZEB1.

Cancer Biology & Therapy
D LiuRob M Ewing

Abstract

Background: Epithelial-to-mesenchymal transition (EMT) is a key step in the transformation of epithelial cells into migratory and invasive tumour cells. Intricate positive and negative regulatory processes regulate EMT. Many oncogenic signalling pathways can induce EMT, but the specific mechanisms of how this occurs, and how this process is controlled are not fully understood. Methods: RNA-Seq analysis, computational analysis of protein networks and large-scale cancer genomics datasets were used to identify ELF3 as a negative regulator of the expression of EMT markers. Western blotting coupled to siRNA as well as analysis of tumour/normal colorectal cancer panels was used to investigate the expression and function of ELF3. Results: RNA-Seq analysis of colorectal cancer cells expressing mutant and wild-type β-catenin and analysis of colorectal cancer cells expressing inducible mutant RAS showed that ELF3 expression is reduced in response to oncogenic signalling and antagonizes Wnt and RAS oncogenic signalling pathways. Analysis of gene-expression patterns across The Cancer Genome Atlas (TCGA) and protein localization in colorectal cancer tumour panels showed that ELF3 expression is anti-correlated with β-catenin and markers of E...Continue Reading

References

Jun 1, 2004·Molecular and Cellular Biology·Jason D PrescottArthur Gutierrez-Hartmann
May 18, 2007·Nature Reviews. Cancer·Héctor PeinadoAmparo Cano
Jan 21, 2009·Cancer Metastasis Reviews·Otto SchmalhoferThomas Brabletz
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michal ShefferEytan Domany
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Julian Heuberger, Walter Birchmeier
Jan 22, 2011·BMC Medical Genomics·Andre LobodaTimothy J Yeatman
Nov 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ester Sánchez-TillóAntonio Postigo
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Sep 14, 2012·G3 : Genes - Genomes - Genetics·Andrew T KwonWyeth W Wasserman
Apr 17, 2013·BMC Bioinformatics·Edward Y ChenAvi Ma'ayan
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Oct 27, 2015·Cancer Letters·Kyung-Won MinSeung Joon Baek
Jan 26, 2016·Cancer Cell·Shinichi YachidaTatsuhiro Shibata
Feb 28, 2016·The Biochemical Journal·Vijaya Narasihma Reddy GajulapalliBramanandam Manavathi
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Dec 21, 2017·Cancers·Trung Vu, Pran K Datta

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Citations

Dec 5, 2019·Frontiers in Genetics·Pablo Ivan Pereira RamosArtur Trancoso L de Queiroz
Feb 24, 2021·Cell Communication and Signaling : CCS·Dan DongWei Wang

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Datasets Mentioned

BETA
GSE95670
GSE41258

Methods Mentioned

BETA
RNA-Seq
scraping
protein assay

Software Mentioned

Enrichr
oPOSSUM
DeSeq
ImageJ
Pathway Studio
MoBaS
TopHat2

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