PMID: 6403625Mar 11, 1983Paper

Elicitation and detection of in vitro H-2-linked Ir gene regulated antibody responses to poly(LTyr, Glu)-poly(DLAla)--poly(LLys)

Journal of Immunological Methods
H Z Weissberger, H B Dickler

Abstract

A microculture system is described in which secreted antibody responses to the synthetic polypeptide (T,G)-A--L were obtained in vitro. Responses were highly reproducible, antigen-dependent, antigen-specific, and under H-2-linked Ir gene control. Critical elements in the system include the schedule of in vivo antigen-priming, removal of the stimulating antigen after 3 days of culture, and a sensitive detection system (double-antibody ELISA). This system should be useful in the analysis of the mechanism of action of Ir genes as well as the mechanisms by which anti-idiotype antibodies modulate immune responses.

References

Nov 1, 1975·The Journal of Experimental Medicine·E Trenkner, R Riblet
Mar 1, 1979·The Journal of Experimental Medicine·P Marrack, J W Kappler
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Cosenza, H Köhler
Jun 1, 1972·The Journal of Experimental Medicine·H O McDevittG D Snell
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Jan 1, 1980·Immunological Reviews·G KelsoeK Rajewsky

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.