Abstract
A microculture system is described in which secreted antibody responses to the synthetic polypeptide (T,G)-A--L were obtained in vitro. Responses were highly reproducible, antigen-dependent, antigen-specific, and under H-2-linked Ir gene control. Critical elements in the system include the schedule of in vivo antigen-priming, removal of the stimulating antigen after 3 days of culture, and a sensitive detection system (double-antibody ELISA). This system should be useful in the analysis of the mechanism of action of Ir genes as well as the mechanisms by which anti-idiotype antibodies modulate immune responses.
References
Nov 1, 1975·The Journal of Experimental Medicine·E Trenkner, R Riblet
Jun 1, 1978·The Journal of Experimental Medicine·P Marrack, J W Kappler
Jun 1, 1978·The Journal of Experimental Medicine·A SingerR J Hodes
Dec 1, 1978·The Journal of Experimental Medicine·J W Kappler, P Marrack
Mar 1, 1979·The Journal of Experimental Medicine·P Marrack, J W Kappler
Dec 1, 1977·European Journal of Immunology·R J Hodes, A Singer
Oct 1, 1977·The Journal of Experimental Medicine·A SingerR J Hodes
Nov 1, 1973·The Journal of Experimental Medicine·J A KappB Benacerraf
Aug 1, 1974·The Journal of Experimental Medicine·J TaussigR Isac
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Cosenza, H Köhler
Apr 1, 1974·European Journal of Immunology·K Eichmann
Jun 1, 1972·The Journal of Experimental Medicine·H O McDevittG D Snell
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Feb 1, 1982·European Journal of Immunology·P I NadlerR J Hodes
Jun 1, 1981·European Journal of Immunology·D H SachsG Miller
Jan 1, 1980·Immunological Reviews·G KelsoeK Rajewsky