Eliciting cytotoxic T lymphocytes against human laryngeal cancer-derived antigens: evaluation of dendritic cells pulsed with a heat-treated tumor lysate and other antigen-loading strategies for dendritic-cell-based vaccination

Journal of Experimental & Clinical Cancer Research : CR
Fan-Qin WeiWei-Ping Wen

Abstract

Dendritic cells (DCs) have been used successfully in clinical pilot studies. However, tumor-specific immunity and clinical responses were only induced in certain cancer patients. It has been well documented that immunotherapy efficacy can be optimized for responses using antigen pulsing. The human laryngeal squamous cell cancer (LSCC) cell line SNU899 was used to evaluate the in vitro anti-tumor efficacy of three different preparations of dendritic cell (DC) vaccines consisting of either whole tumor cells or their derivatives including: i) DCs pulsed with a tumor cell supernatant (DC-TCS), ii) DCs pulsed with whole-cell tumor stressed lysate (DC-TSL), and iii) DCs pulsed with irradiated tumor cells (DC-ITC). Our results showed that DC-TSL is an effective source of tumor-associated antigens (TAAs) for pulsing DCs. DC-TSL induced the highest expansion of TAA-specific T cells, the strongest Th1 cytokine response, and the most potent cytotoxic T lymphocyte (CTL) activity. DC-TCS and DC-ITC inhibited T cell activation but induced a certain extent of CTL activity. These data suggest that DC-TSL is a more potent inducer of antitumor immunity against laryngeal cancer than other antigen-loading strategies using whole tumor cell material...Continue Reading

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Citations

Sep 14, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yohei TakedaTsukasa Seya
Oct 27, 2018·Journal of Cellular Physiology·Adeleh TaghikhaniSeyyed-Mohammad Moazzeni
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Sep 19, 2020·Cells·Adrian von WitzlebenChristian H Ottensmeier
Oct 14, 2021·Cancer Immunology, Immunotherapy : CII·Xueying WangSusheng Miao

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry

Software Mentioned

SPSS
FACSDiva

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