Elicitor hydrophobin Hyd1 interacts with Ubiquilin1-like to induce maize systemic resistance

Journal of Integrative Plant Biology
Chuanjin YuJie Chen

Abstract

Trichoderma harzianum is a plant-beneficial fungus that secretes small cysteine-rich proteins that induce plant defense responses; however, the molecular mechanism involved in this induction is largely unknown. Here, we report that the class II hydrophobin ThHyd1 acts as an elicitor of induced systemic resistance (ISR) in plants. Immunogold labeling and immunofluorescence revealed ThHyd1 localized on maize (Zea mays) root cell plasma membranes. To identify host plant protein interactors of Hyd1, we screened a maize B73 root cDNA library. ThHyd1 interacted directly with ubiquilin 1-like (UBL). Furthermore, the N-terminal fragment of UBL was primarily responsible for binding with Hyd1 and the eight-cysteine amino acid of Hyd1 participated in the protein-protein interactions. Hyd1 from T. harzianum (Thhyd1) and ubl from maize were co-expressed in Arabidopsis thaliana, they synergistically promoted plant resistance against Botrytis cinerea. RNA-sequencing analysis of global gene expression in maize leaves 24 h after spraying with Curvularia lunata spore suspension showed that Thhyd1-induced systemic resistance was primarily associated with brassinosteroid signaling, likely mediated through BAK1. Jasmonate/ethylene (JA/ET) signaling...Continue Reading

References

Apr 29, 2000·Trends in Plant Science·N D PaulJ E Taylor
Oct 12, 2001·Trends in Endocrinology and Metabolism : TEM·C Müssig, T Altmann
Feb 23, 2002·Molecular Plant-microbe Interactions : MPMI·Jurriaan TonCorné M J Pieterse
Mar 12, 2004·Annual Review of Phytopathology·L C van LoonC M Pieterse
Mar 24, 2004·Nature Reviews. Microbiology·Gary E HarmanMatteo Lorito
Dec 2, 2004·Acta Microbiologica Et Immunologica Hungarica·L KredicsElisabeth Nagy
Oct 13, 2005·FEMS Microbiology Reviews·Markus B LinderMerja E Penttilä
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·A H Y KwanJ P Mackay
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Slavica DjonovićCharles M Kenerley
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Dec 23, 2008·Fungal Genetics and Biology : FG & B·C PliegoC Ramos
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Sep 15, 2009·Trends in Plant Science·Delphine ChinchillaBirgit Kemmerling
Oct 22, 2009·The Plant Journal : for Cell and Molecular Biology·Qing LuYuhai Cui
May 12, 2010·Annual Review of Phytopathology·Matteo LoritoEnrique Monte
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Jan 11, 2011·Current Opinion in Microbiology·Cécile Segonzac, Cyril Zipfel
Sep 17, 2011·Nature Reviews. Microbiology·Irina S DruzhininaChristian P Kubicek
Oct 15, 2011·Microbiology·Rosa HermosaEnrique Monte
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Catherine AlbrechtCyril Zipfel
Jun 14, 2012·PLoS Pathogens·Jagadeesh BayryJean-Paul Latgé
Feb 27, 2013·Frontiers in Plant Science·Jyoti Shah, Jürgen Zeier
Apr 17, 2013·Molecular Plant·Dong-Lei YangZuhua He
Jun 20, 2013·Phytopathology·Shahin S AliFiona M Doohan
Jul 23, 2013·Current Opinion in Plant Biology·Aardra Kachroo, Guillaume P Robin
Aug 7, 2013·Annual Review of Phytopathology·Prasun K MukherjeeCharles M Kenerley
Apr 29, 2014·Molecular Cell·Alberto P Macho, Cyril Zipfel
Oct 16, 2014·Molecular Plant-microbe Interactions : MPMI·Michelina RuoccoMatteo Lorito
Feb 15, 2015·Molecular & Cellular Proteomics : MCP·Netta-Li LamdanBenjamin A Horwitz
Mar 6, 2015·Methods in Molecular Biology·Yun Jin, Hui-Shan Guo
Apr 30, 2015·Trends in Plant Science·Andrea BalmerBrigitte Mauch-Mani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.