Elicitor stimulation of the defense response in cultured plant cells monitored by fluorescent dyes

Archives of Biochemistry and Biophysics
P. S. Low, P. F. Heinstein

Abstract

Addition of fungal elicitors to plant cells in suspension is known to stimulate biochemical changes in the plant cell leading to production of defense compounds. In this paper we demonstrate that introduction of elicitors from the pathogenic fungus Verticillium dahliae to cultured cotton, tobacco, or soybean cells leads to a rapid, dramatic change in the fluorescence of several membrane-associated potentiometric or pH-sensitive dyes. The fluorescence transitions occur abruptly following a brief (0 to 10 min) lag period in apparently most cells of the suspension simultaneously. Furthermore, both the length of the lag period and the rate of the subsequent fluorescence change were shown to be highly dependent on elicitor concentration. When the crude elicitor extract was separated by gel filtration chromatography into several active fractions, the ability of each fraction to stimulate phytoalexin production in the cotton cell suspension was found to correlate directly with the rate of the fluorescence decrease in the fluorescence assay. Because the assay is rapid, simple to perform, quantitative, and reproducible, it represents an attractive alternative to the more cumbersome and perhaps less quantitative elicitor assays currently...Continue Reading

References

Jan 1, 1979·Annual Review of Biophysics and Bioengineering·A S Waggoner
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Citations

Oct 1, 1991·Journal of Chemical Ecology·F P Neupane, D M Norris
Mar 15, 1996·Biochimica Et Biophysica Acta·S C DwyerT L Leto
Oct 9, 1998·Progress in Lipid Research·E Blée
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E E FarmerC A Ryan
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Chandra, P S Low
Mar 1, 2006·Molecular Plant Pathology·Emilie F Fradin, Bart P H J Thomma
Aug 8, 1997·Biochemical and Biophysical Research Communications·A T Schroeder Taylor, P S Low

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