Elicitors' influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions

Applied Microbiology and Biotechnology
Tanya BiswasArchana Mathur

Abstract

Cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of Pseudomonas monteili, Bacillus circularans, Trichoderma atroviridae, and Trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of Panax quinquefolius. Abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. Cobalt nitrate (50 μM) increased total ginsenosides content by twofold (54.3 mg/L) within 5 days. It also induced the Rc synthesis that was absent in the control cultures. Elicitation with P. monteili (2.5 % v/v, 5 days) also supported 2.4-fold enhancement in saponin yield. Elicitation by T. atroviridae or hydrogen peroxide induced the synthesis of Rg3 and Rh2 that are absent in ginseng roots. The highest ginsenosides productivity (3.2-fold of control) was noticed in cells exposed to 1.25 % v/v dose of T. atroviridae for 5 days. Treating cells with T. harzianum for 15 days afforded maximum synthesis and leaching (8.1 mg/L) of ginsenoside Rh1.

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Citations

Jan 30, 2018·Biotechnology and Applied Biochemistry·Jun LuWenyuan Gao
Jan 20, 2018·Applied Microbiology and Biotechnology·Kim-Cuong LeSo-Young Park
Mar 25, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Samrin ShaikhVinay Kumar
Aug 1, 2020·Chemistry & Biodiversity·Baobao QiangYing Gao
Jun 3, 2021·Pathogens·Daniele Cristina FontanaDurval Dourado Neto
Jul 20, 2021·Critical Reviews in Food Science and Nutrition·Priyanka ChopraRavi Gupta
Aug 14, 2021·Acta Pharmaceutica Sinica. B·Maoqi HouZhengtao Wang
Sep 2, 2021·Applied Microbiology and Biotechnology·Christoph Wawrosch, Sergey B Zotchev

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