Eliminating false positive C4 sugar tests on New Zealand Manuka honey

Rapid Communications in Mass Spectrometry : RCM
Karyne M RogersJulie Cox

Abstract

Carbon isotope analyses (delta(13)C) of some New Zealand Manuka honeys show that they often fail the internationally recognised Association of Official Analytical Chemists sugar test (AOAC method 998.12) which detects added C(4) sugar, although these honeys are from unadulterated sources. Failure of these high value products is detrimental to the New Zealand honey industry, not only in lost export revenue, but also in brand and market reputation damage. The standard AOAC test compares the carbon isotope value of the whole honey and corresponding protein isolated from the same honey. Differences between whole honey and protein delta(13)C values should not be greater than +1.0 per thousand, as it indicates the possibility of adulteration with syrups or sugars from C(4) plants such as high fructose corn syrup or cane sugar.We have determined that during the standard AOAC method, pollen and other insoluble components are isolated with the flocculated protein. These non-protein components have isotope values which are considerably different from those of the pure protein, and can shift the apparent delta(13)C value of protein further away from the delta(13)C value of the whole honey, giving a false positive result for added C(4) sug...Continue Reading

Citations

Apr 30, 2014·Journal of Applied Microbiology·F KhanS van Vuuren
Sep 3, 2013·Food Chemistry·Russell FrewRobert Van Hale
Feb 27, 2014·Journal of Agricultural and Food Chemistry·Karyne M RogersPam Rogers
Jan 23, 2014·Journal of Agricultural and Food Chemistry·Karyne M RogersMerilyn Manley-Harris

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