Elimination of chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid from infected chrysanthemum by cryopreservation

Protoplasma
Su Min JeonChang Kil Kim

Abstract

Chrysanthemum morifolium 'Borami' and 'Secret Pink' showing symptoms of stunt disease caused by chrysanthemum stunt viroid (CSVd) and 'Yellow Cap' showing chlorotic mottle disease caused by chrysanthemum chlorotic mottle viroid (CChMVd) were confirmed to be infected by the respective viroids by using reverse transcription polymerase chain reaction (RT-PCR). Real-time PCR results showed that the viroid concentrations in the infected cultivars varied between the different regions of origin (Chilgok, Gumi, and Gyeongsan). We applied a cryopreservation protocol for elimination of CSVd from naturally infected 'Borami' collected from Gumi, showing the lowest concentration of CSVd, by varying several factors such as plant vitrification solutions (PVS2 and PVS3), duration of exposure to liquid nitrogen, shoot-tip size, and low-temperature treatment. The solution (PVS2) and low-temperature treatment were found to be critical factors determining the efficacy of viroid elimination. We optimized the protocol by combining of all resulted optimal factors and tested the applicability of the protocol in 'Borami' collected from Chilgok and Gyeongsan and in 'Secret Pink' from Chilgok, Gumi, and Gyeongsan, which displayed different viroid concent...Continue Reading

References

Oct 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T O Diener
Feb 16, 2002·The EMBO Journal·José-Antonio Daròs, Ricardo Flores
Sep 21, 2010·Biotechnology Advances·Chaohong FengQiaochun Wang

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Citations

Apr 9, 2019·Plant Disease·Lei ZhaoQiaochun Wang
Nov 9, 2019·Plant Methods·Jing-Wei LiQiao-Chun Wang
Dec 20, 2018·Viruses·Tomoyuki NabeshimaMunetaka Hosokawa

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