PMID: 8971007Dec 1, 1996Paper

Elimination of hepatitis C virus RNA in infected human hepatocytes by adenovirus-mediated expression of ribozymes

Journal of Virology
A LieberM A Kay

Abstract

Hepatitis C virus (HCV), a positive-strand RNA virus, is the major infectious agent responsible for causing chronic hepatitis. Currently, there is no vaccine for HCV infection, and the only therapy for chronic hepatitis C is largely ineffective. To investigate new genetic approaches to the management of HCV infection, six hammerhead ribozymes directed against a conserved region of the plus strand and minus strand of the HCV genome were isolated from a ribozyme library, characterized, and expressed from recombinant adenovirus vectors. The expressed ribozymes individually or in combination were efficient at reducing or eliminating the respective plus- or minus-strand HCV RNAs expressed in cultured cells and from primary human hepatocytes obtained from chronic HCV-infected patients. This study demonstrates the potential utility of ribozyme therapy as a strategy for the treatment of hepatitis C virus infection.

References

Mar 1, 1992·Journal of Virology·K Tsukiyama-KoharaA Nomoto
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F NegroF Bonino
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Nov 1, 1992·The Journal of Infectious Diseases·J T WangD S Chen
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J H HanM Houghton
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Jun 1, 1983·Analytical Biochemistry·D M Durnam, R D Palmiter
Jan 17, 1994·The American Journal of Medicine·G L Davis
Jan 1, 1995·Molecular and Cellular Biology·A Lieber, M Strauss
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A WeinerC M Walker
Nov 1, 1994·Current Opinion in Oncology·E Poeschla, F Wong-Staal
Nov 1, 1994·The Journal of Investigative Dermatology·S M Sullivan
Oct 1, 1993·European Journal of Biochemistry·A LieberM Strauss
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y K ShimizuH Yoshikura
Jan 1, 1993·Methods in Enzymology·A LieberM Strauss
May 1, 1996·Journal of Virology·A Lieber, M A Kay

❮ Previous
Next ❯

Citations

Mar 22, 2001·Journal of Internal Medicine·A M Larson, R L Carithers
Dec 11, 2002·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Betsy T KrenClifford J Steer
Jul 5, 2005·Expert Opinion on Investigational Drugs·J WuM A Zern
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M KrugerF Wong-Staal
Aug 6, 1999·Antisense & Nucleic Acid Drug Development·J Albuquerque-SilvaS Houard
Aug 2, 2002·Journal of Pediatric Gastroenterology and Nutrition·Humberto E Soriano, Markus Grompe
Nov 9, 2005·Microscopy Research and Technique·Srikanta DashRobert Garry
Mar 18, 2004·Oligonucleotides·Camille Martinand-MariIan Robbins
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D G MacejakL M Blatt
May 17, 2003·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·David SteeleGarrett A Soukup
Dec 22, 2007·Expert Opinion on Investigational Drugs·Gurjot Kaur, Ipsita Roy
Jan 22, 2003·Journal of Gastroenterology and Hepatology·Robert Y M ChenStephen A Locarnini
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·S HuangG Nemerow
Mar 19, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Chandan GuhaJayanta Roy-Chowdhury
Jul 3, 2004·Journal of Viral Hepatitis·G V Papatheodoridis, E Cholongitas
Oct 16, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Jianlin GengZhigang Tian
Aug 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F von WeizsäckerH E Blum
Sep 20, 2002·Journal of Virology·Bruno B BordierJeffrey S Glenn
Apr 22, 2005·Biological Chemistry·Cristina Romero-LópezAlfredo Berzal-Herranz
Apr 17, 2003·FEMS Microbiology Reviews·Elena Puerta-FernándezAlfredo Berzal-Herranz
Dec 16, 2020·Gene Therapy·Burak BerberCihan Tastan
Sep 24, 1999·Biochemical and Biophysical Research Communications·T HeintgesJ R Wands
Feb 8, 2000·Blood Cells, Molecules & Diseases·T J ShenC Ho
Nov 20, 1998·Current Opinion in Biotechnology·P J WelchF Wong-Staal
Oct 26, 1999·Drug Discovery Today·M A Walker
Aug 27, 1999·Molecular Medicine Today·H R Rosen, D R Gretch
Nov 10, 1998·Trends in Biotechnology·B BramlageF Eckstein
May 20, 1999·Antiviral Research·V N LazarevB S Naroditsky
Apr 2, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kyung-Ju RyuSeong-Wook Lee
Jan 11, 2000·Clinical Microbiology Reviews·P LeyssenJ Neyts
Apr 15, 2003·Clinics in Liver Disease·Raffaele De Francesco, Charles M Rice
Jun 27, 2009·Pharmacology & Therapeutics·Claire GondeauPatrick Maurel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.