Elimination of "kitome" and "splashome" contamination results in lack of detection of a unique placental microbiome.

BMC Microbiology
Isoken Nicholas OlomuMartha H Mulks

Abstract

A placental microbiome, which may be altered in gestational diabetes mellitus (GDM), has been described. However, publications raising doubts about the existence of a placental microbiome that is different than contaminants in DNA extraction kits and reagents ("kitomes") have emerged. The aims of this study were to confirm the existence of a placental microbiome distinct from contaminants and determine if it is altered in GDM mothers. We first enrolled normal weight, obese and GDM mothers (N = 17) at term elective cesarean section delivery in a pilot case control study. Bacterial DNA was extracted from placental parenchyma, maternal and cord blood, maternal vaginal-rectal swabs, and positive and negative controls with the standard Qiagen/MoBio Power Soil kit. Placentas had significantly higher copies of bacterial 16S rRNA genes than negative controls, but the placental microbiome was similar in all three groups and could not be distinguished from contaminants in blank controls. To determine the source and composition of the putative placental bacterial community identified in the pilot study, we expanded the study to 10 subjects per group (N = 30) and increased the number and variety of negative controls (N = 53). We modified o...Continue Reading

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Citations

Aug 23, 2020·Nutrients·Francisco J Pérez-Cano
Jan 14, 2021·Microbiome·Jens Walter, Mathias W Hornef
Jan 14, 2021·Microbiome·Rachel B Silverstein, Indira U Mysorekar
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Viola SennTanja Restin
Mar 3, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L BrubakerA J Wolfe
May 12, 2021·Nature Microbiology·Katherine M KennedyThorsten Braun
Jun 1, 2021·Frontiers in Immunology·Cristina KalbermatterStephanie C Ganal-Vonarburg
Aug 17, 2021·Bioscience Reports·Karen GrewalPhillip R Bennett
Sep 30, 2021·Microbiology Spectrum·Scott J Dos SantosUNKNOWN Maternal Microbiome Legacy Project Team
Oct 2, 2021·Scientific Reports·Jenni TurunenTerhi Tapiainen
Nov 11, 2021·Expert Review of Molecular Diagnostics·Leonard SchueleNatacha Couto
Nov 18, 2021·Critical Reviews in Microbiology·Richard CariñoMasahiro Umezaki

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Methods Mentioned

BETA
cesarean section
PCR

Software Mentioned

PERMANOVA
Plac
Illumina Real Time Analysis ( RTA )
Paleontological Statistics Software Package for Education and ...
SigmaPlot
Mothur
PAST3 (
ANOSIM
QIIME2
Illumina Bcl2fastq

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