Elimination of mouse tumor cells from neonate spermatogonial cells utilizing cisplatin-entrapped folic acid-conjugated poly(lactic-co-glycolic acid) nanoparticles in vitro

International Journal of Nanomedicine
Ronak ShabaniMorteza Koruji

Abstract

Some male survivors of childhood cancer are suffering from azoospermia. In addition, spermatogonial stem cells (SSCs) are necessary for the improvement of spermatogenesis subsequent to exposure to cytotoxic agents such as cisplatin. The aim of this study was to evaluate the anticancer activity of cisplatin-loaded folic acid-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on mouse malignant cell line (EL4) and SSCs in vitro. SSCs were co-cultured with mouse malignant cell line (EL4) cells and divided into four culture groups: 1) control (cells were co-cultured in the culture medium), 2) co-cultured cells were treated with cisplatin (10 μg/mL), 3) co-cultured cells were treated with cisplatin-loaded folic acid-conjugated PLGA NPs, and 4) co-cultures were treated with folic acid-conjugated PLGA for 48 hours. The NPs were prepared, characterized, and targeted with folate. In vitro release characteristics, loading efficiency, and scanning electron microscopy and transmission electron microscopy images were studied. Cancer cells were assayed after treatment using flow cytometry and TUNEL assay. The co-cultures of SSCs and EL4 cells were injected into seminiferous tubules of the testes after treating with cis-diami...Continue Reading

Citations

Apr 19, 2019·Current Opinion in Endocrinology, Diabetes, and Obesity·Omar AbdelaalHooman Sadri-Ardekani
May 9, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Muhammad Asim FarooqBo Wang
Jun 12, 2021·International Journal of Pharmaceutics·Tugce BoztepeIgnacio E León
Aug 2, 2021·Methods : a Companion to Methods in Enzymology·Ana Luiza LimaGuilherme M Gelfuso
Dec 4, 2021·Frontiers in Physiology·Barbara FraserRobert J Aitken

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Methods Mentioned

BETA
biopsy
infrared spectroscopy
dynamic light scattering
scanning electron microscopy
reverse transcription PCR
PCR
flow cytometry
electron microscopy
FACS

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