PMID: 200907Oct 1, 1977

Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage

Proceedings of the National Academy of Sciences of the United States of America
W E StewartK Cantell

Abstract

Human leukocyte interferon (HuLeIF) preparations contain distinct molecular forms of interferon exhibiting significant heterogeneties in sizes when analyzed by electrophoresis in sodium dodecyl sulfate (NaDodSO(4))/polyacrylamide gels, migrating in two broad bands of activity with peaks at about 21,000 and 15,000 daltons. HuLeIFs exhibit extensive charge heterogeneities when analyzed by isoelectric focusing, resolving into several major peaks of approximately equal activity distributed from pH 5.7 to 7.0. When HuLeIF preparations are treated with 0.01 M sodium periodate buffer, pH 4.5, at 4 degrees , both the size and charge heterogeneities rapidly disappear: periodate-treated HuLeIF migrates as a single, narrow band at 15,000 daltons in NaDodSO(4)/polyacrylamide gels and focuses as a single, narrow band at pH 5.7. Quantitative considerations suggest that either the larger, heterogeneously charged HuLeIFs are converted to the smaller, size- and charge-homogeneous interferon by extensive chemical deglycosylation, or, alternatively, the smaller, 15,000 dalton, pH 5.7 interferon is much more stable to periodate treatment than are the other interferon forms. However, the activity of each of the variously charged forms of HuLeIF iso...Continue Reading

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E A HavellJ Vilcek
May 1, 1977·The Journal of General Virology·K PauckerC A Ogburn
Mar 4, 1977·Annals of the New York Academy of Sciences·S PestkaJ Vilcek
Feb 1, 1977·Antimicrobial Agents and Chemotherapy·T C CesarioJ G Tilles
Sep 2, 1976·The New England Journal of Medicine·H B GreenbergT C Merigan
Feb 11, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Knight
Jul 18, 1975·Biochemical and Biophysical Research Communications·F H Reynolds, P M Pitha
Sep 1, 1974·Virology·W E Stewart
May 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·V G EdyP De Somer

Citations

Feb 15, 2001·Biopolymers·S Pestka
Sep 18, 1977·Blut·H Strander
Jan 1, 1980·Archives of Virology·K C ChadhaE Sulkowski
Jan 1, 1981·Pharmacology & Therapeutics·V Bocci
Jan 1, 1985·Pharmacology & Therapeutics·S Pestka, S J Tarnowski
Jan 1, 1985·Pharmacology & Therapeutics·J A Langer, S Pestka
Oct 1, 1981·Human Immunology·P E Andreotti, P Cresswell
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W E StewartM Wiranowska-Stewart
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M N ThangC Chany
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L A PickeringW E Stewart
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J S TwuR H Schloemer
Jan 1, 1979·Preparative Biochemistry·E Bollin, E Sulkowski
Feb 1, 1981·Journal of Interferon Research·I A BraudeW E Stewart
Apr 3, 2009·Cellular Immunology·Simone SponzaS Landolfo
Aug 1, 1981·Archives of Biochemistry and Biophysics·M RubinsteinS Pestka
Oct 15, 1978·Virology·R RaghowD W Kingsbury
Jun 1, 1984·European Journal of Cancer & Clinical Oncology·H K Kotlar
Jan 1, 1980·Annals of the New York Academy of Sciences·L A PickeringW E Stewart
Jun 29, 1978·Biochemical and Biophysical Research Communications·S L Abreu, F C Bancroft
Feb 1, 1986·Scandinavian Journal of Immunology·A RambaldiA Mantovani
Jan 1, 1980·Scandinavian Journal of Immunology·K Berg, I Heron
Oct 1, 1982·Medicinal Research Reviews·Z E Dziewanowska, S Pestka
May 1, 1988·European Journal of Haematology·C G PetersonP Venge
Jan 1, 1982·Journal of Interferon Research·D A Eppstein

Related Concepts

SDS-PAGE
Interferons
Isoelectric Focusing
Leukocytes
Oxidation-Reduction
Para-Influenza Virus Type 1
Periodic Acid (HIO4)

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