ELISA assay for measurement of human hemoglobin A and hemoglobin F

American Journal of Clinical Pathology
M T Makler, A J Pesce

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the measurement of nanogram quantities of hemoglobin A and hemoglobin F is described. The assay employs hemoglobin bound to polystyrene test tubes. The antigen is quantitated by the addition of rabbit antibody specific for the hemoglobin type, followed by a second antibody of goat antirabbit gamma globulin coupled to alkaline phosphatase. Quantitation of the reaction is achieved by incubating the coated tubes with p-nitrophenyl phosphate. The binding characteristics, the linearity, the ability of the assay to discriminate between hemoglobin A and hemoglobin F, the ability to discriminate between native and denatured gamma globin, and the comparison of this assay with a previously described immunoradiometric (IRMA) assay are discussed.

Citations

Mar 1, 1982·Critical Reviews in Clinical Laboratory Sciences·W L Marsh, H M Koenig
Jul 10, 2018·Journal of Immunoassay & Immunochemistry·Sasiwan KerdpooThanusak Tatu
Apr 2, 2008·IUBMB Life·Laura Manca, Bruno Masala
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·L A KowalskiJ A Grasso

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