PMID: 7539443Mar 1, 1995Paper

ELISA for anti-HCV antibody employing a shorter synthetic core region peptide

Journal of Virological Methods
I Nakagiri, K Ichihara

Abstract

A new ELISA for anti-HCV antibody was developed employing a shorter synthetic N-terminal peptide, 2-62aa, within the core region of 1-191aa. The basic performance of the assay was comparable to three other second-generation assays using longer HCV core antigens. To evaluate assay performance at the borderline level, 25 samples with indeterminate results were selected from 3000 routine serum samples. Only 5 of the 25 sera were found to be HCV-RNA-positive by a nested PCR assay and with apparent clinical evidence of HCV infection. The results of the new ELISA agreed with those of the PCR-RNA test in 23/25 (kappa statistics 0.75), whereas C22-3 of the RIBA II test using 2-120aa of the core agreed in 9/25 (0.09), the Abbott pHCV-34 EIA test using 1-150aa agreed in 10/25 (-0.12), and a neutralization inhibition assay for Abbott EIA II using 2-120aa agreed in 6/25 (0.02). These results indicate that the UBI CORE ELISA has greatly improved specificity and can be a useful indication of viremia in HCV infection.

References

Oct 30, 1990·Biochemical and Biophysical Research Communications·K MuraisoK Shimotohno
Jun 1, 1991·Journal of Virology·S HaradaT Miyamura
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M S NasoffA M Prince
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J ChibaT Miyamura
Apr 21, 1990·Lancet·S KanekoN Hattori
Aug 4, 1990·Lancet·J P Clewley
Oct 6, 1990·Lancet·J A GarsonR S Tedder

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