ELISA for the routine determination of antitoxic immunity to tetanus

Journal of Biological Standardization
O SimonsenI Heron

Abstract

Serum samples from 727 persons with different vaccination histories were assessed for tetanus antitoxin content in an enzyme linked immunosorbent assay (ELISA) and tested for tetanus toxin neutralization activity in mice in order to compare the results obtained by the two methods. Neutralizing antibody activities in sera from individuals previously completely vaccinated correlated well with results obtained by ELISA and the accuracy increased with increasing antitoxin concentration in serum. This correlation was observed in sera from persons vaccinated recently as well as in sera from persons vaccinated many years ago. In sera from persons with an incomplete vaccination history ELISA was found to be an unreliable tool for the prediction of in vivo results. Many of these sera had antitoxin levels by ELISA far above the in vivo values, probably due to the presence of non specific or low avidity antitoxin which is detected in ELISA. The lowest ELISA value reliably predictive of protective antibody activity in serum irrespective of vaccination history was found to be 0.16 IU/ml. It was concluded that ELISA is useful for larger population studies as an initial test, but sera with an antitoxin content below 0.16 IU/ml should also be ...Continue Reading

Citations

Aug 13, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K CaglarC Aybay
Jan 24, 1991·Journal of Immunological Methods·J Rungsiwongse, K Ratanabanangkoon
Jul 1, 1987·Journal of Biological Standardization·M German-FattalA German
Apr 1, 1987·Journal of Biological Standardization·O SimonsenI Heron
Apr 1, 1989·Journal of Biological Standardization·C F HendriksenJ G Kreeftenberg
Dec 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C F Hendriksen
Jan 1, 1990·Biologicals : Journal of the International Association of Biological Standardization·J LyngL Ljungqvist
Mar 2, 2005·The Journal of Adolescent Health : Official Publication of the Society for Adolescent Medicine·William M CassidyCharmaine Venters
Oct 24, 2000·Journal of Immunological Methods·J WaloryW Hryniewicz
Mar 4, 2000·The Journal of Emergency Medicine·F M AbrahamianR W Carlson
Oct 1, 1991·Biologicals : Journal of the International Association of Biological Standardization·J Lyng, I Heron
Jun 1, 1992·Biologicals : Journal of the International Association of Biological Standardization·H Aggerbeck, I Heron
Mar 23, 1995·The New England Journal of Medicine·P J GergenG Virella
Nov 9, 2007·The New England Journal of Medicine·Ian J AmannaMark K Slifka
Aug 31, 2006·The Pediatric Infectious Disease Journal·Milagritos D TapiaMyron M Levine
Jan 2, 2007·European Journal of Emergency Medicine : Official Journal of the European Society for Emergency Medicine·Muriel StubbePhilippe E Lheureux
Sep 3, 2011·Southern Medical Journal·Peter AtaroShagufta Ahsan
Dec 11, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M KristiansenI Heron
Jul 23, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Shams, I Heron
Aug 25, 2012·Biomarkers in Medicine·Joanna Z PengJohn A Wagner
Feb 18, 2009·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Patricia PalmeiraMagda M S Carneiro-Sampaio
Feb 11, 2005·Annals of Tropical Medicine and Parasitology·E M Redwan, M K El-Awady
Jul 22, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Elena DanilovaHaakon Sjursen
Oct 22, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Shahana A Choudhury, Fazle Matin
Oct 6, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Solvor Berntsen StølevikBerit Granum
Jan 27, 2015·Médecine et maladies infectieuses·M SchlumbergerB Tep

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