ELISA inhibition method in detection of mite and chironomid antigens in environmental samples of dust, soil and air

Allergy
J Y KimuraA Ishii

Abstract

To determine the amount of chironomids and mite antigens in the indoor and outdoor environment, we investigated samples from air, soil and house dust in the area around Lake Kojima, located in the western part of Japan. Three species of chironomid (Tokunagayusurika akamusi, Chironomus yoshimatsui and Chironomus plumosus) and species of mite, Dermatophagoides farinae antigens were studied. The antigens were detected and quantified by inhibition ELISA. In the outdoor environment, C. yoshimatsui and T. akamusi antigens were more abundant than mite antigen, and seasonal fluctuations were observed. In the indoor environment, mite was the predominant antigen. D. farinae antigen was detected in almost all dust samples, with a slight reduction in winter compared to summer.

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Citations

Dec 1, 2001·Annual Review of Entomology·Larry G Arlian
Apr 1, 1995·Japanese Journal of Medical Science & Biology·H MatsuokaY Chinzei
Oct 3, 1999·The Korean Journal of Parasitology·T S YongH S Park
Jun 26, 2007·International Archives of Allergy and Immunology·Cheol-Woo KimChein-Soo Hong
Jul 25, 2019·Allergy, Asthma & Immunology Research·Myung Hee YiTai Soon Yong

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