ELISA measurement of specific antibodies to phosphorylated tau in intravenous immunoglobulin products

International Immunopharmacology
David A LoefflerMary P Coffey

Abstract

The therapeutic effects of intravenous immunoglobulin (IVIG) products were recently studied in Alzheimer's disease (AD) patients. Pilot studies produced encouraging results but phase II and III trials gave disappointing results; a further study is in progress. IVIG products contain antibodies to tau protein, the main component of neurofibrillary tangles (NFTs). The tau used to detect IVIG's anti-tau antibodies in previous studies was non-phosphorylated recombinant human tau-441, but NFT-associated tau is extensively phosphorylated. The objective of this study was to determine if various IVIG products contain specific antibodies to phosphorylated tau (anti-pTau antibodies). ELISAs were used to evaluate binding of six IVIG products to a 12 amino acid peptide, tau 196-207, which was phosphorylated ("pTau peptide") or non-phosphorylated ("non-pTau peptide") at Serine-199 and Serine-202. Both amino acid residues are phosphorylated in AD NFTs. Each IVIG's "anti-pTau antibody ratio" was calculated by dividing its binding to the pTau peptide by its binding to the non-pTau peptide. Seven experiments were performed and data were pooled, with each experiment contributing one data point from each IVIG product. Mean anti-pTau antibody ratio...Continue Reading

References

Jun 15, 2007·The Journal of Biological Chemistry·Diane P HangerBrian H Anderton
Jul 20, 2010·Journal of Neuroimmunology·Paul SzaboNorman Relkin
May 15, 2013·International Immunopharmacology·Lynnae M SmithDavid A Loeffler
Mar 25, 2014·Journal of Neuroinflammation·Isabelle St-AmourFrédéric Calon
May 27, 2014·International Immunopharmacology·Lynnae M SmithDavid A Loeffler

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