PMID: 38284Jan 1, 1979

ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes

Journal of Immunological Methods
B M Gray

Abstract

A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.

References

Jan 1, 1985·Journal of Clinical Immunology·R L GregoryJ R McGhee
Apr 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R R ReinertA Kaufhold
Oct 10, 1985·Journal of Immunological Methods·A SuttonR Schneerson
Nov 25, 1988·Journal of Immunological Methods·A BarraJ L Preud'homme
Mar 21, 1991·Journal of Immunological Methods·S H FengK E Stein
Jun 24, 1991·Journal of Immunological Methods·S RuthsT A Out
Jan 4, 1993·Journal of Immunological Methods·B M Gray, D R Shaw
Jun 1, 1995·Veterinary Immunology and Immunopathology·K NielsenW Kelly
Mar 1, 1984·Human Immunology·J F SchwaberH Lazarus
Oct 1, 1988·Epidemiology and Infection·B M GrayH C Dillon
Jun 26, 2009·Journal of the American Chemical Society·Ravindra A De SilvaPeter R Andreana
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C E RubensD L Kasper
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsD L Kasper
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsD L Kasper
Sep 1, 1991·Parasite Immunology·J E LillywhiteA E Bianco
Dec 17, 1983·British Medical Journal·F Di PadovaF Harder
Mar 1, 1995·Journal of Clinical Pathology·A E AgbarakweH M Chapel
Mar 1, 1985·The Journal of Clinical Investigation·P F KohlerL L Heston
Apr 16, 2013·Journal of Immunological Methods·Robert W KaminskiEdwin V Oaks

Citations

Related Concepts

Study, Methodological
Acclimatization
Gene Products, Protein
Adsorption
Lysine
Polysaccharides, Bacterial
Streptococcus agalactiae
Antigens
Plastics
Enzyme-Linked Immunosorbent Assay

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.