ELISA to measure neutralizing capacity of anti-C1-inhibitor antibodies in plasma of angioedema patients

Journal of Immunological Methods
Ruchira EngelDörte Hamann

Abstract

Neutralizing autoantibodies (NAbs) against plasma serpin C1-inhibitor (C1-inh) are implicated in the rare disorder, acquired angioedema (AAE). There is insufficient understanding of the process of antibody formation and its correlation with disease progression and severity. We have developed an ELISA for detecting neutralizing capacity of anti-C1-inh positive plasma samples that can be used to study changes in NAb repertoire in patient plasma over the course of disease. The ELISA is based on the specific interaction of active C1-inh with its target protease C1s. Decrease in the amount of C1s bound to immobilized C1-inh in the presence of test samples is proportional to the neutralizing capacity of the sample. Assay specificity, intra- and inter-assay variation and assay cut-off are determined using anti-C1-inh antibodies. Assay capability is demonstrated using plasma samples from AAE patients. The assay is specific to a neutralizing anti-C1-inh antibody and shows no interference by a non-neutralizing anti-C1-inh antibody or by the plasma matrix. Intra-assay and inter-assay variations are determined as 17 and 18% respectively. Neutralizing capacity of antibody positive AAE patient plasma samples (n=16) with IgG or IgM type antib...Continue Reading

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Citations

May 5, 2016·Clinical Reviews in Allergy & Immunology·Henriette FarkasLilian Varga
Nov 9, 2018·Frontiers in Immunology·Kristina N EkdahlBo Nilsson
Nov 2, 2019·Transfusion Medicine Reviews·Lillemor Skattum

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