ELISpot assay as a sensitive tool to detect cellular immunity following influenza vaccination in kidney transplant recipients

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Monika LindemannH Grosse-Wilde

Abstract

Enhanced cellular immunity following influenza vaccination has been undetectable in kidney transplant recipients so far. Protection from influenza is dependent on cellular and humoral immunity. Aim of the study was to investigate immune responses before and after vaccination with influenza A and B antigens in 65 kidney transplant recipients. A significant increase in proliferative responses was only observed towards influenza B (P < 0.0001) by lymphocyte transformation test. The enzyme-linked immunospot (ELISpot) assay was more sensitive and detected significant, 3- to 5-fold increases (P < 0.0001) in interferon-gamma secretion using influenza A and B antigens. Furthermore, influenza antibody titers increased significantly (P < 0.0001). At month 1 post-vaccination 85% of patients displayed specific cellular, and 95% or 92% humoral immunity against influenza A and B, respectively. Thus, applying the sensitive ELISpot assay, influenza-specific cellular immunity could be detected for the first time in kidney transplant recipients after vaccination.

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Citations

Jan 31, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rebecca Pellett MadanBetsy C Herold
Sep 3, 2010·Transplantation·Monika LindemannOliver Witzke
Aug 13, 2010·Kidney International·Geertrude H StruijkFrederike J Bemelman
Nov 4, 2008·Human Immunology·Monika LindemannHans Grosse-Wilde
Jun 21, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L C WillcocksK G C Smith
Jan 13, 2011·Scandinavian Journal of Immunology·M LindemannO Witzke
Dec 24, 2009·Journal of Medical Virology·J AllmendingerU G Liebert
Apr 23, 2014·Transplant Infectious Disease : an Official Journal of the Transplantation Society·H J JeonJ Yang
Mar 31, 2015·Transplant Infectious Disease : an Official Journal of the Transplantation Society·A AmbatiP Ljungman
Sep 12, 2015·Saudi Journal of Kidney Diseases and Transplantation : an Official Publication of the Saudi Center for Organ Transplantation, Saudi Arabia·Reza Karbasi-AfsharHossein Khedmat
Oct 7, 2008·Journal of Immunological Methods·Therese VallerskogChristina Trollmo
Dec 14, 2018·International Journal of Molecular Sciences·Monika LindemannBenjamin Wilde

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