PMID: 11334981May 4, 2001Paper

ELISPOT assay to measure antigen-specific murine CD8(+) T cell responses

Journal of Immunological Methods
Luzia H CarvalhoFidel Zavala

Abstract

The enzyme-linked immunospot technique (ELISPOT) relies on the visualization of cytokine secretion by individual T cells following in vitro stimulation with antigen. This assay has been developed and standardized for the quantitative detection of antigen-specific CD8(+) T cells in mice subjected to different immunization protocols [J. Immunol. Methods 181 (1995) 45]. We have identified important variables that affect the efficacy of the ELISPOT assay and in this protocol we describe this methodology in detail. As a model, we used the production of interferon-gamma by CD8(+) T cells from peripheral blood, spleen and liver of mice immunized with malaria sporozoites expressing the H-2K(d)-restricted SYVPSAEQI. This protocol has also been used successfully to detect Th1 and Th2 epitope specific CD4(+) T cells.

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Citations

Jul 4, 2009·Cancer Gene Therapy·M Rodrigo-GarzónG González-Aseguinolaza
Mar 17, 2004·The Journal of Experimental Medicine·Annika VogtErvin H Epstein
Jan 20, 2005·The Journal of Experimental Medicine·Deng TaoRuth S Nussenzweig
Jan 23, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Yaoxing HuangDavid D Ho
Apr 24, 2014·Human Immunology·Govinda Sharma, Robert A Holt
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Julius C R HafallaFidel Zavala
Sep 26, 2008·Science in China. Series C, Life Sciences·Jin LüXiLiang Wang
Nov 22, 2014·European Journal of Immunology·Nicolas RochereauStéphane Paul
Apr 1, 2004·International Journal of Cancer. Journal International Du Cancer·Iñigo TirapuIgnacio Melero
Feb 26, 2011·Immunological Reviews·Julius Clemence HafallaKai Matuschewski
Jun 16, 2010·Experimental Dermatology·Usriansyah HadisEva M Kovacs
Oct 16, 2009·European Journal of Immunology·Michael G OverstreetFidel Zavala
Mar 22, 2005·Journal of Immunological Methods·Chan ZengElizabeth J McFarland
Sep 30, 2017·Cells·Josué da Costa Lima-JuniorFátima Conceição-Silva
Apr 7, 2006·European Journal of Immunology·Julius C R HafallaAna Rodriguez
Dec 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sheila T TangTom H M Ottenhoff
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ian A CockburnFidel Zavala
Sep 16, 2020·Nature Methods·Andrew W WoodhamHidde L Ploegh
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julius C R HafallaFidel Zavala

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