PMID: 8614640Apr 1, 1996Paper

Elk-1 can recruit SRF to form a ternary complex upon the serum response element

Nucleic Acids Research
B V LatinkićL F Lau

Abstract

The initial genomic response to serum growth factors is the transcriptional activation of a set of immediate-early genes. Serum-induced transcriptional activation of several of these genes involves the formation of a ternary complex that includes the serum response factor (SRF), a 62 kDa ternary complex factor (TCF) and a serum response element (SRE). TCF alone does not bind the SRE of the protooncogene c-fos, but requires the prior assembly of the SRF-SRE binary complex for it to be recruited into a ternary complex. Here we show that this SRF-SRE binary complex is not an obligatory prerequisite for the formation of a serum responsive ternary complex. We demonstrate that Elk-1, which has properties of TCF can recruit SRF into a ternary complex on elements that do not support formation of the SRF-DNA binary complex. We also show that for two immediate-early genes, pip92 and nur77, formation of the ternary complex may occur without the prior assembly of SRF-DNA binary complex. Finally, we show that the ability of different sequences to support formation of Elk-l-SRF-DNA ternary complex in vitro correlates with their ability to respond to serum growth factors in vivo. Our results suggest that a much broader range of DNA sequences ...Continue Reading

References

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Citations

Jun 30, 1998·Trends in Biochemical Sciences·B WasylykA Gutierrez-Hartmann
May 7, 1998·The International Journal of Biochemistry & Cell Biology·A D SharrocksP R Yates
Feb 11, 2004·Nature Immunology·Patrick S CostelloRichard Treisman
Jun 19, 2001·The EMBO Journal·M Hassler, T J Richmond
Nov 2, 2005·Molecular and Cellular Biology·Jiliang ZhouB Paul Herring
Nov 30, 2005·Molecular and Cellular Biology·Gilles BuchwalterBohdan Wasylyk
Mar 22, 2012·BMC Molecular Biology·Magdalena FlorkowskaAneta Kasza
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Eunha KimSeyun Kim
Aug 24, 2010·Biochimica Et Biophysica Acta·Paulina WyrzykowskaAneta Kasza
Dec 13, 2006·Journal of Neurochemistry·Jung Bum ParkKwang Chul Chung
Jul 28, 2011·Journal of Cellular Physiology·Roger M IlaganJohn W Ludlow
Sep 27, 2013·Critical Reviews in Biochemistry and Molecular Biology·Adwitiya Kar, Arthur Gutierrez-Hartmann
May 18, 2016·Reviews in the Neurosciences·Andrew L EagleAlfred J Robison
Oct 19, 2004·Oncogene·Min-Hyuk YooJae-Hong Kim
Nov 9, 2004·The Journal of Biological Chemistry·Aneta KaszaAndrew D Sharrocks
Jul 30, 2019·Wellcome Open Research·Ian Prise, Andrew D Sharrocks
Jan 21, 2016·The Journal of Biological Chemistry·Eswar ShankarDavid Danielpour
Aug 18, 2021·Transcription·John G TooleyChristine E Schaner Tooley

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