Elk-1, C/EBPalpha, and Pit-1 confer an insulin-responsive phenotype on prolactin promoter expression in Chinese hamster ovary cells and define the factors required for insulin-increased transcription

The Journal of Biological Chemistry
K K Jacob, F M Stanley

Abstract

The transcription factor(s) that mediate insulin-increased gene transcription are not well defined. These studies use phenotypic conversion of Rat2 and Chinese hamster ovary (CHO) cells with transcription factors to identify components required for regulation of prolactin promoter activity and its control by insulin. The pituitary-derived GH4 cells contain all of the transcription factors required for insulin-increased prolactin-chloramphenicol acetyltransferase (CAT) expression while HeLa cells require only Pit-1, a pituitary-specific factor. However, Rat2 and CHO cells require additional factors. We had determined previously that the transcription factor that mediates insulin-increased prolactin gene expression was likely an Ets-related protein. Elk-1 and Sap-1 were the only Ets-related transcription factors tested as chimeras with LexA DNA-binding domain that were able to mediate insulin-increased expression of a LexA-CAT reporter plasmid. Elk-1 and Sap-1 are expressed in GH4 and HeLa cells but Rat2 and CHO cells express Sap-1, but not Elk-1. Expression of Elk-1 made Rat2 cells (but not CHO cells) insulin responsive. C/EBPalpha also binds to the prolactin promoter at a sequence overlapping the binding site for Elk-1. Express...Continue Reading

References

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Citations

Jul 18, 2009·Molecular Endocrinology·Ushma R JagFrederick M Stanley
Oct 1, 2013·Molecular and Cellular Endocrinology·Muktar A Mahajan, Frederick M Stanley
Dec 26, 2001·Endocrinology·Dawn L Duval, Arthur Gutierrez-Hartmann
Mar 29, 2002·The Journal of Biological Chemistry·Anthony Igor Vulin, Frederick M Stanley
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fumihiko TakeshitaKen J Ishii

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