Abstract
Magnetic nanoparticles (MNPs) functionalized with targeting moieties can recognize specific cell components and induce mechanical actuation under magnetic field. Their size is adequate for reaching tumors and targeting cancer cells. However, due to the nanometric size, the force generated by MNPs is smaller than the force required for largely disrupting key components of cells. Here, we show the magnetic assembly process of the nanoparticles inside the cells, to form elongated aggregates with the size required to produce elevated mechanical forces. We synthesized iron oxide nanoparticles doped with zinc, to obtain high magnetization, and functionalized with the epidermal growth factor (EGF) peptide for targeting cancer cells. Under a low frequency rotating magnetic field at 15 Hz and 40 mT, the internalized EGF-MNPs formed elongated aggregates and generated hundreds of pN to dramatically damage the plasma and lysosomal membranes. The physical disruption, including leakage of lysosomal hydrolases into the cytosol, led to programmed cell death and necrosis. Our work provides a novel strategy of designing magnetic nanomedicines for mechanical destruction of cancer cells.
Citations
Mar 16, 2018·Advanced Materials·Congyu WuYu Cheng
Jan 25, 2018·Biomaterials Science·Madiha SaeedAiguo Wu
Aug 25, 2018·Electromagnetic Biology and Medicine·Meng ZhaXin Zhang
Oct 9, 2018·Small·Yang ChenMaoquan Chu
Dec 24, 2019·Small·Mengwei ChenYu Cheng
May 21, 2020·Advanced Materials·Meihua XieBin He
Sep 26, 2020·Nanoscale·George OpletalAmanda S Barnard
Mar 3, 2018·Nanotechnology·K SpyridopoulouO Kalogirou
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Abdel Rahman Abdel Fattah, Adrian Ranga
Jul 1, 2020·Advanced Science·Jiaojiao WuYu Cheng
Jul 31, 2019·Chemical Science·Arghya BandyopadhyaySayan Bhattacharyya
Jul 27, 2017·Annals of Biomedical Engineering·Yajing ShenGustavo R Plaza
Apr 3, 2019·Annual Review of Neuroscience·Michael G ChristiansenPolina Anikeeva
Sep 24, 2019·Future Medicinal Chemistry·Raul OrtizJose Prados
Mar 20, 2019·Pharmaceutics·Courtney van BallegooieDonald T Yapp
Jan 30, 2021·Nature Materials·Jung-Uk LeeJinwoo Cheon
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Henry Ruiz-GarciaDaniel M Trifiletti
Mar 20, 2021·ACS Applied Materials & Interfaces·Aleksey A NikitinMaxim A Abakumov
Jul 6, 2020·Journal of Colloid and Interface Science·Tanja GoršakDarko Makovec
Jun 26, 2021·Colloids and Surfaces. B, Biointerfaces·A R IliasovM A Abakumov
Apr 1, 2020·ACS Nano·Xian WangYu Sun
Jul 19, 2017·ACS Applied Materials & Interfaces·Liuyang ZhangXianqiao Wang
Aug 31, 2021·Journal of Imaging·River GassenKathrin Spendier
Nov 23, 2021·ACS Nano·Yajing ShenYu Cheng