ELTD1 Function in Hepatocellular Carcinoma is Carcinoma-Associated Fibroblast-Dependent

Journal of Cancer
Anna KanRong-Ping Guo

Abstract

Introduction: EGF, latrophilin, and seven transmembrane domain containing 1 (ELTD1) constitutes an orphan G-protein-coupled receptor (GPCR) of the adhesion family. High expression of ELTD1 is correlated with favorable prognosis of hepatocellular carcinoma (HCC). After silencing ELTD1 expression, however, tumor invasiveness is drastically reduced. The underlying mechanism of this apparent contradictory phenomenon is unknown. Because adhesion GPCRs couple extracellular adhesion to intracellular signaling, as a member of this family, ELTD1 function may be related to its tumor microenvironment. We therefore investigated the interaction between ELTD1 and the HCC tumor microenvironment. Methods: ELTD1 expression was assessed by immunohistochemical analyses of tissue samples from two independent groups of 333 patients with HCC. Correlations between the ELTD1 expression and the clinicopathological values were examined. We also constructed ELTD1 overexpression and knockdown HCC cell lines and conducted a series of in vivo and in vitro ELTD1 functional assays. We further collected carcinoma associated fibroblast (CAF) culture supernatants to culture HCC cell lines and repeat the respective functional assays in comparison with the control...Continue Reading

Citations

Mar 31, 2020·International Journal of Cancer. Journal International Du Cancer·Marine JaryJean P Feugeas
Jun 3, 2021·International Journal of Molecular Sciences·Ani-Simona SevastreAnica Dricu
Aug 6, 2021·Molecular Cancer Research : MCR·Helen SheldonAdrian L Harris

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Methods Mentioned

BETA
electrophoresis
PCR
xenograft
xenografts

Software Mentioned

SPSS

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