Elucidating mechanisms of thermostabilization of poliovirus by D2O and MgCl2

Archives of Biochemistry and Biophysics
C H ChenR Crainic

Abstract

To understand a significant reduction in the loss of poliovirus infectivity by D2O and a combination of D2O and MgCl2 at 37-45 degrees C, this paper attempts to elucidate the mechanisms underlying the thermostabilization of poliovirus. Three serotypes of Sabin oral poliovirus vaccine strains were investigated. Temperature-dependent fluorescence emission intensity studies showed that the effects of D2O and MgCl2 on the stability and conformation of poliovirus are correlated with those of the infectivity of poliovirus. Fluorescence steady-state polarization revealed that the conformation of poliovirus capsid is sensitive to D2O medium and MgCl2 salt, and that the rigidity of poliovirus conformation is increased in their presence. The exposure of poliovirus tryptophan residues to water is modified by D2O and MgCl2, as evidenced by changes in fluorescence emission intensity excited at 295 nm. The involvement of hydrogen bonding in the D2O effect was demonstrated by the greatly increased value of relative fluorescence intensity. Conformational alteration was also shown by changes in the positive band (193-230 nm) of circular dichroism spectra. D2O and MgCl2 were also found to reduce the interaction of virus with water as examined by...Continue Reading

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Citations

Nov 28, 2013·Journal of Pharmaceutical Sciences·Wei QiC Russell Middaugh
Jul 6, 2014·Biologicals : Journal of the International Association of Biological Standardization·Ozan S KumruDavid B Volkin
Sep 20, 2017·Human Vaccines & Immunotherapeutics·Gaurav KanojiaGideon Kersten
Oct 30, 2009·Expert Review of Vaccines·Arnab SenRaj Kumar Singh
Mar 3, 2004·The Journal of General Virology·Estela Escribano-RomeroVictoria Ley
May 1, 2017·The Journal of Chemical Physics·Arup K Pathak, Tusar Bandyopadhyay
May 18, 2020·AAPS PharmSciTech·Yajie ZhangDebadyuti Ghosh
Jan 24, 1998·Archives of Biochemistry and Biophysics·C H ChenD L Martin
Jan 22, 2003·Biochimica Et Biophysica Acta·Chang Hwei ChenWilfredo Colón
Mar 11, 2003·Biomacromolecules·Dan L SackettRalph Nossal

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